摘要
目的比较地氟烷与七氟烷麻醉对睡眠剥夺小鼠睡眠质量的影响。方法清洁级健康雄性C57BL/6小鼠32只,10周龄,体质量20~25 g,采用随机数字表法分为4组(n=8):对照组(C组)、睡眠剥夺组(SD组)、睡眠剥夺+七氟烷组(SD+SEV组)和睡眠剥夺+地氟烷组(SD+DES组)。4组小鼠均进行脑电-肌电电极埋置手术,适应7 d后采用毛刷轻柔刺激法小鼠完全睡眠剥夺12 h(6:00—18:00)。睡眠剥夺后6 h分为2个时间节段:T_(1)期2 h(18:00—20:00)和T_(2)期4 h(20:00—24:00)。T_(1)期:SD组睡眠剥夺后自然睡眠,C组和SD组均吸入60%氧气1.5 L/min。SD+DES组和SD+SEV组睡眠剥夺后暴露于6%地氟烷或2.5%七氟烷2 h,60%氧气1.5 L/min。T_(2)期:4组小鼠均自然睡眠,同时记录脑电-肌电信号,采用Lunion Data软件计算觉醒(WAKE)时间、快动眼睡眠(REM)时间和非快动眼睡眠(NREM)时间占总时间的百分比和次数。结果与C组比较,SD组、SD+SEV组和SD+DES组12 h睡眠剥夺期间,NREM和REM时间百分比降低,WAKE时间百分比升高(P<0.05);与T_(1)期比较,SD组T_(2)期NREM时间百分比升高,WAKE和REM时间百分比降低(P<0.05);与SD组比较,SD+SEV组和SD+DES组T_(2)期NREM和REM时间百分比降低,WAKE时间百分比升高(P<0.05);SD+SEV组和SD+DES组T_(2)期NREM、REM和WAKE时间百分比差异无统计学意义(P>0.05);与SD+SEV组比较,SD+DES组T_(2)期NREM次数减少(P<0.05)。结论地氟烷麻醉改善睡眠剥夺小鼠睡眠质量的效应优于七氟烷麻醉。
Objective To compare the effects of desflurane and sevoflurane anesthesia on the sleep quality of sleep-deprived mice.Methods Thirty-two clean-grade healthy male C57BL/6 mice,aged 10 weeks,weighing 20-25 g,were divided into 4 groups(n=8 each)by the random number table method:control group(C group),sleep deprivation group(SD group),sleep deprivation+sevoflurane group(SD+SEV group),and sleep deprivation+desflurane group(SD+DES group).In the four groups,EEG-EMG electrodes were implanted for recording EEG and EMG,and sleep deprivation model was developed by the gentle stimulation method with a brush for 12 h(6:00-18:00)after 7 days of adaptation.The 6 h after sleep deprivation was divided into 2 time periods:T_(1) period(18:00-20:00)and T_(2) period(20:00-24:00).T_(1) period In SD group,mice were allowed ad libitum recovery sleep after sleep deprivation.C group and SD group were exposed to 60%oxygen 1.5 L/min.In SD+DES group and SD+SEV group,mice were exposed to 6%desflurane and 2.5%sevoflurane,respectively,for 2 h in 60%oxygen 1.5 L/min following sleep deprivation.T_(2) period Four groups were allowed ad libitum recovery sleep with the EEG-EMG signal recording.The percentages and number of wakefulness time,rapid eye movement time and non-rapid eye movement time during each time period were calculated using Lunion Data software.Results Compared with C group,the percentage of non-rapid eye movement time and the percentage of rapid eye movement time were significantly decreased,and the percentage of wakefulness time was increased during 12 h sleep deprivation in SD group,SD+SEV group and SD+DES group(P<0.05).Compared with T_(1) period,the percentage of non-rapid eye movement time was significantly increased,and the percentage of wakefulness time and percentage of rapid eye movement time were decreased in T_(2) period in SD group(P<0.05).Compared with SD group,the percentage of non-rapid eye movement time and percentage of rapid eye movement time were significantly decreased,and the percentage of wakefulness time was in
作者
张学敏
朱进飘
孙嘉慧
贾俊可
王杰
张宗泽
陈畅
Zhang Xuemin;Zhu Jinpiao;Sun Jiahui;Jia Junke;Wang Jie;Zhang Zongze;Chen Chang(Department of Anesthesiology,Zhongnan Hospital of Wuhan University,Wuhan 430071,China;Wuhan Institute of Physics and Mathematics,Innovation Academy for Precision Measurement Science and Technology,Wuhan 430071,China)
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2023年第2期166-169,共4页
Chinese Journal of Anesthesiology
基金
国家自然科学基金(81671060,81771160)。
关键词
地氟烷
七氟醚
麻醉
睡眠剥夺
睡眠障碍
Desflurane
Sevoflurane
Anesthesia
Sleep deprivation
Sleep disorders
