摘要
建立睡莲花HPLC指纹图谱,并对5种成分进行含量测定,结合化学模式识别法,综合分析各产区睡莲花药材的成分差异,为睡莲花药材的进一步开发利用奠定基础。采用Waters C_(18)色谱柱(250 mm×4.6 mm, 5μm),以乙腈(A)-0.2%磷酸水溶液(B)为流动相,梯度洗脱,流速1.0 mL/min,柱温30℃,检测波长273 nm、350 nm。在273 nm下标定23个共有峰,指认了5种化学成分,350 nm标定17个共有峰,指认了4种化学成分。对15批样品聚类分析,在273 nm下分为4类,350 nm下分为3类。正交偏最小二乘法判别分析(OPLS-DA)共找到273 nm的10个可能影响睡莲花药材质量的差异性标志物。15批样品相似度为0.972~0.999,表明不同批次睡莲花样品存在差异。该方法可有效评价不同产地睡莲花药材的质量差异,为其质量控制提供参考。
HPLC fingerprint of Nymphaeae Flos was established,the content of five components was determined,and the composition differences of Nymphaeae Flos in different origins were comprehensively analyzed by combining with chemical pattern recognition method,which laid the foundation for further development and utilization of Nymphaeae Flos.The HPLC fingerprint was established by Waters C 18(250 mm×4.6 mm,5μm)column.The mobile phase was acetonitrile-0.2%phosphoric acid aqueous solution.The flow rate was 1.0 mL/min.The column temperature was 30℃.The detection wavelength were 273 nm,350 nm.At 273 nm,23 common peaks were identified and five chemical components were identified.At 350 nm,17 common peaks were identified and four chemical components were identified.In the cluster analysis,15 batches of samples were divided into five groups at 273 nm and three groups at 350 nm.Orthogonal partial least squares discriminant analysis(OPLS-DA)identified 10 different markers at 273 nm that could affect the quality of Nymphaeae Flos.The similarity of 15 batches of Nymphaeae Flos samples was 0.972 to 0.999,indicating that different batches of Nymphaeae Flos samples were different.This method can effectively evaluate the quality difference of Nymphaeae Flos from different habitats and provide reference for its quality control.
作者
李智
赵璐
米尔扎提·麦麦提
马璇
LI Zhi;ZHAO Lu;MIERZHATI·Maimaiti;MA Xuan(Xinjiang Cimu Pharmaceutical Research Institute(Co.,Ltd.);Xinjiang Key Laboratory of Generic Technology of Traditional Chinese Medicine(Ethnic Medicine)Pharmacy,Urumqi 830011,China)
出处
《天然产物研究与开发》
CAS
CSCD
2023年第5期828-836,共9页
Natural Product Research and Development
基金
中国民族医药学会科研项目(2019KYXM-M245-117)
自治区“十四五”重大科技专项“安胃疡等地产中药民族药大品种的培育及产业化”课题(2022A03003-5)。
关键词
雪白睡莲
HPLC指纹图谱
相似度
聚类分析
主成分分析
正交偏最小二乘判别分析
Nymphaeae Flos
HPLC fingerprint
similarity
cluster analysis
principal component analysis
orthogonal partial least squares discriminant analysis
