摘要
目的研究鞣酸小檗碱在特应性皮炎(atopic dermatitis,AD)中的治疗作用。方法将30只雄性、SPF级BALB/c小鼠随机分为5组:对照组、模型组、1%鞣酸小檗碱组、5%鞣酸小檗碱组、0.03%他克莫司组,除对照组外其余组于第1周第1、3、5天分别在小鼠双侧耳廓外侧皮肤局部涂抹1%2,4-二硝基氯苯(DNCB)的丙酮-橄榄油溶液各20μL进行初次致敏,第2~3周起每3 d在小鼠双侧耳廓外侧的致敏处涂抹0.5%DNCB的丙酮-橄榄油溶液各20μL进行再次致敏,并每天分别使用药物进行治疗;对照组于相同时间涂抹等量的丙酮-橄榄油基质。每周按相同时间间隔测量两次小鼠双侧耳部厚度。实验结束后处死小鼠,切取小鼠耳朵行组织病理学检查。结果经过DNCB致敏之后,与对照组小鼠相比,模型组小鼠耳部厚度显著增加,差异有显著统计学意义(P<0.01)。经过1%、5%鞣酸小檗碱和0.03%他克莫司治疗后,小鼠耳部厚度有明显改善,与模型组相比,差异均有显著统计学意义(P<0.01),且各浓度鞣酸小檗碱组与0.03%他克莫司组相比,小鼠耳部厚度差异无统计学意义(P>0.05)。组织病理学结果显示模型组与对照组相比,小鼠耳部真皮层水肿、炎症细胞浸润明显,表皮层厚度增加,其厚度差异具有显著统计学意义(P<0.01)。使用1%、5%鞣酸小檗碱和0.03%他克莫司治疗后,表皮层厚度减少,差异有显著统计学意义(P<0.01),说明疾病状况改善,各浓度鞣酸小檗碱组与0.03%他克莫司组相比差异无统计学意义(P>0.05)。结论鞣酸小檗碱对特应性皮炎具有良好的治疗效果,有望用于开发治疗AD,为临床治疗AD提供新思路。
Objective To study the therapeutic effect of berberine tannin in atopic dermatitis(AD).Methods Thirty male,pathogen-free BALB/c mice were randomly divided into 5 groups:the model group,1%berberine tannate group,5%berberine tannate group,and 0.03%tacrolimus group were treated on the 1st,3rd,and 5th day of the first week on the outer skin of the bilateral auricles of mice respectively apply 20μL each of 1%DNCB(2,4-dinitrochlorobenzene)in acetone-olive oil for initial sensitization.From the 2nd to the 3rd week,20μL of acetone-olive oil solution of 0.5%DNCB was applied to the sensitized parts of the bilateral auricles of the mice every 3 days for resensitization,and the mice were treated with drugs every day.The control group applied the same amount of acetone-olive oil base at the same time.Bilateral ear thickness of mice was measured twice a week at the same time interval.After the experiment,the mice were sacrificed,and the ears of the mice were excised for histopathological examination.Results After DNCB sensitization,compared with the control group,the ear thickness of the model group was significantly increased,and there was a statistically significant difference(P<0.01).After the treatment of 1%,5%berberine tannate and 0.03%tacrolimus,the ear thickness of mice was significantly improved,and compared with the model group,there were significant statistical differences(P<0.01).Compared with the 0.03%tacrolimus group,there was no significant difference in the ear thickness of mice between the berberine tannate groups at different concentrations(P>0.05).Histopathological results showed that compared with the control group,the model group had obvious edema and inflammatory cell infiltration in the ear dermis,and the thickness of the epidermis increased,and the difference in thickness was statistically significant(P<0.01).After treatment with 1%,5%berberine tannin and 0.03%tacrolimus,the thickness of the epidermis was reduced,and there was a significant statistical difference(P<0.01),indicating that the disease con
作者
马银超
门月华
初明
王子元
黄国强
江麟
鲁锡霖
黄祺甯
王月丹
李燕
高翔
王丽珺
刘媛
裴军
初正云
贾冬
MA Yinchao;MEN Yuehua;CHU Ming;WANG Ziyuan;HUANG Guoqiang;JIANG Lin;LU Xilin;HUANG Qining;WANG Yuedan;LI Yan;GAO Xiang;WANG Lijun;LIU Yuan;PEI Jun;CHU Zhengyun;JIA Dong(Liaoning University of Traditional Chinese Medicine,Shenyang 110847,Liaoning,China;Peking University Third Hospital,Beijing 100191,China;Peking University Health Science Center,Beijing 100191,China)
出处
《辽宁中医药大学学报》
CAS
2023年第4期21-24,F0003,共5页
Journal of Liaoning University of Traditional Chinese Medicine
基金
国家自然科学基金项目(81603119)
北京市自然科学基金项目(7174316)
北京大学医学交叉研究种子基金项目(BMU2021MX2021)。
