摘要
目的探索莓茶提取物对2型糖尿病(type 2 diabetes mellitus,T2DM)大鼠糖脂代谢及肝脏沉默信息调节因子1(silence information regulator 1,SIRT1)、AMP活化蛋白激酶(AMP activated protein kinase,AMPK)、过氧化物酶体增殖物激活受体γ辅助活化因子1α(peroxisome proliferator-activated receptor-γcoactivator-1α,PGC-1α)蛋白表达的影响。方法高脂高糖喂养联合链脲佐菌素(35 mg/kg)腹腔注射建立T2DM大鼠模型,建模成功后随机分为模型组、莓茶低剂量组(0.4 g/kg)、莓茶高剂量组(0.8 g/kg),另设普通饲料喂养大鼠为正常组,每组8只。各组灌胃给药6周后,行口服葡萄糖耐量试验计算血糖-时间曲线下面积(area under the curve,AUC),ELISA法检测空腹血胰岛素(fasting insulin,FINS)水平,计算稳态模型以评估胰岛素抵抗指数(homeostatic model assessment for insulin resistance,HOMA-IR),全自动生化分析仪测定血清总胆固醇(total cholesterol,TC)、甘油三酯(triglycerides,TG)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)、超氧化物歧化酶(superoxide dismutase,SOD)、丙二醇(malondialdehyde,MDA)水平,HE染色观察肝脏组织形态学变化,Western blot检测肝脏SIRT1、AMPK、PGC-1α蛋白表达。结果与正常组比较,模型组FBG、AUC、FINS、HOMA-IR、TC、TG、LDL-C、MDA均明显升高(P<0.01),HDL-C、SOD和SIRT1、AMPK、PGC-1α蛋白表达均明显降低(P<0.01),肝脏组织肝索排列紊乱,肝细胞间隙不清晰,呈脂肪变性改变。与模型组比较,莓茶低剂量组、莓茶高剂量组FBG、FINS、HOMA-IR、TG、MDA均明显降低(P<0.05,P<0.01),SOD和SIRT1、AMPK、PGC-1α蛋白表达均明显升高(P<0.05,P<0.01),肝脏组织肝索排列相对整齐,脂肪变性得到改善;莓茶高剂量组AUC、TC均明显降低(P<0.05),HDL-C明显升高(P<0.05)。与莓茶低剂量组比较,莓茶高剂量组FBG、AUC、FINS、TC、MDA均明显降低(P<0.05),S
Objective To explore the effects of ampelopsis grossedentata extract on glycolipid metabolism and silence information regulator 1(SIRT1),AMP activated protein kinase(AMPK)and peroxisome proliferator-activated receptor-γcoactivator 1-α(PGC-1α)expressions of liver in type 2 diabetes mellitus(T2DM)rats.Methods T2DM rat model was established by high-fat/high-sugar diet combined with streptozotocin injection(STZ,35 mg/kg)intraperitoneally.Then,T2DM rats were randomly divided into model group,low-dose ampelopsis grossedentata extract group(0.4 g/kg),and high-dose ampelopsis grossedentata extract group(0.8 g/kg),with 8 rats in each one.In addition,normal diet rats(n=8)were set as the normal group.After 6 weeks of gavage administration,oral glucose tolerance tests were performed to calculate the area under the curve(AUC)of glucose-time,fasting insulin(FINS)levels were measured by ELISA,and homeostatic model assessment for insulin resistance(HOMA-IR)was performed.Total cholesterol(TC),triglycerides(TG),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C),superoxide dismutase(SOD)and malondialdehyde(MDA)levels were measured by automated biochemical analyzer.The morphological changes of liver were observed by HE staining,and the protein expressions of SIRT1,AMPK and PGC-1αin liver tissues were measured by Western blot.Results Compared with normal group,fasting blood glucose(FBG),AUC,FINS,HOMA-IR,TC,TG,LDL-C and MDA levels dramatically increased in model group rats(P<0.01),the expressions of HDL-C,SOD,SIRT1,AMPK and PGC-1αdecreased significantly(P<0.01),the arrangement of hepatic cord in liver tissue was disordered,the hepatic intercellular space was unclear and hepatic steatosis was exhibited.Compared with model group,FBG,FINS,HOMA-IR,TG and MDA significantly decreased in low-and high-dose ampelopsis grossedentata extract groups(P<0.05,P<0.01),while the expressions of SOD,SIRT1,AMPK and PGC-1αsignificantly increased(P<0.05,P<0.01),the hepatic cord arrangement was relatively neat,and h
作者
肖颖馥
王能
盛文
刘露梅
孙天松
李波男
何清湖
XIAO Yingfu;WANG Neng;SHENG Wen;LIU Lumei;SUN Tiansong;LI Bonan;HE Qinghu(College of Chinese Medicine,Hunan University of Chinese Medicine,Changsha,Hunan 410208,China;Laboratory of Andrology,Hunan University of Chinese Medicine,Changsha,Hunan 410208,China;College of Integrated Chinese and Western Medicine,Hunan University of Chinese Medicine,Changsha,Hunan 410208,China;Hunan University of Medicine,Huaihua,Hunan 418000,China)
出处
《湖南中医药大学学报》
CAS
2023年第5期807-813,共7页
Journal of Hunan University of Chinese Medicine
基金
全国中医药高等教育“十四五”规划教育科研课题(ZD-20-11)
湖南中医药大学研究生创新课题项目(2021CX07)
湖南中医药大学-湖南中和大汉健康产业运营管理有限公司联合基金项目(院发〔2022〕14号)。
关键词
2型糖尿病
莓茶提取物
氧化应激
沉默信息调节因子1
AMP活化蛋白激酶
type 2 diabetes mellitus
ampelopsis grossedentata extract
oxidative stress
silence information regulator 1
AMP activated protein kinase
