摘要
以来源于我国12个省份49个羊肚菌样品为试验材料,采用ISSR-PCR分子标记法,研究我国羊肚菌不同地区栽培品种间的遗传关系和多样性,以期明确我国现有羊肚菌栽培品种之间的遗传关系,为羊肚菌核心种质资源开发及遗传育种提供参考依据。结果表明:最优的ISSR-PCR反应体系为10×PCR Buffer(Mg^(2-))为2.5μL,Mg^(2+)为20 mmol·L^(-1),DNA为1μg, dNTP Mix为1μL,Tap酶为0.4μL,引物为1μL,ddH_(2)O补至25μL。运用筛选出的5条ISSR引物进行羊肚菌的多态性分析,共获得111个电泳条带,其中110个为多态性条带,多态比率为99.10%。采用UPGMA法进行聚类分析,在相异系数为0.72时,可以将49个样品划分为3个大的类群。综上,我国羊肚菌栽培品种之间有较为丰富的遗传多样性。
The inter-simple sequence repeat(ISSR)molecular markers were used to explore the genetic diversity and genetic relationship of natural populations of 49 Morchella samples from 12province regions.The aim of this study was to reveal the genetic diversity of Morchellaresources,and analyze their genetic differences,so as to provide reference for the selection and breeding of Morchella resources.The results showed that the optimal ISSR-PCR reaction system was as follows 10×PCR Buffer(Mg^(2-))was 2.5μL,Mg^(2+)was 20mmol·L^(-1),DNA was 1μg,dNTP Mix was 1μL,Taq enzyme was 0.4μL,primer was 1μL,ddH_(2)O supplemented to 25μL.and a total of 111bands were amplified with 5primers,including 110polymorphic bands(PPB),The proportion of PPB was 99.10%.The UPGMA method was used for cluster analysis,and 49samples could be divided into three large groups when the number of different lines was 0.72.In conclusion,there is abundant genetic diversity among cultivated varieties of Morchellain China.
作者
吕锐玲
陈实
王杰
胡俊俊
郑永良
LYU Ruiling;CHEN Shi;WANG Jie;HU Junjun;ZHENG Yongliang(College of Biology and Agricultural Resources,Huanggang Normal University/Hubei Key Laboratory for the Improvement of Economic Forest Germplasm and Comprehensive Utilization of Resources/Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains,Huanggang,Hubei 438000)
出处
《北方园艺》
CAS
北大核心
2023年第8期107-114,共8页
Northern Horticulture
基金
湖北省重点研发计划资助项目(2020BBB091)
经济林木种质改良与资源综合利用湖北省重点实验室开放课题资助项目(2021ZD09)
黄冈师范学院博士科研启动基金资助项目(2042020014)。