摘要
目的探究晚期糖基化终末产物(Receptor for Advanced Glycation End Products,RAGE)受体阻断剂对癫痫幼鼠脑电图、神经元损伤及S100B蛋白表达的影响。方法选取40只无特定病原体级SD雄性幼鼠,随机分为正常组(N组)、模型组(M组)、苯妥英钠组(P组)、RAGE受体阻断剂组(R组),每组10只。M组、P组、R组腹腔注射戊四氮建立癫痫模型,N组不建立该模型,建模成功后,对P组腹腔注射20 mg/kg苯妥英钠,对R组腹腔注射1 mg/kg RAGE受体阻断剂FPS-ZM,N组、M组同期腹腔注射同体积0.9%氯化钠溶液。观察各组幼鼠模型行为学,记录脑电图,采用FJB染色和尼氏染色检测神经元损伤情况,免疫酶标法检测S100B蛋白表达。结果与N组比较,M组、P组、R组幼鼠Racine评分、发作持续时间、发作次数明显升高(P<0.05);与M组比较,P组、R组幼鼠Racine评分、发作持续时间、发作次数明显降低(P<0.05);且与P组相比,R组上述指标显著降低(P<0.05)。与N组比较,M组、P组、R组幼鼠波幅明显升高(P<0.05);频率明显降低(P<0.05);与M组比较,P组、R组幼鼠波幅明显降低(P<0.05),频率明显升高(P<0.05);且与P组相比,R组上述指标变化显著(P<0.05)。与M组相比,P组、R组神经元损伤明显减轻,神经元数量有所增加,神经元形态有所改善,神经元细胞及尼氏小体数目明显增加。与N组比较,M组、P组、R组海马、中脑、额叶皮层S100B蛋白表达显著升高(P<0.05);与M组比较,P组、R组海马、中脑、额叶皮层S100B蛋白表达显著降低(P<0.05);且与P组相比,R组上述指标显著降低(P<0.05)。结论RAGE受体阻断剂可显著改善癫痫幼鼠脑电图和神经元损伤,并抑制S100B蛋白表达。
Objective To investigate the effects of receptor for advanced glycation end products(RAGE)receptor blocker on EEG,neuron injury and S100B protein expression in epileptic young rats.Methods Forty specific pathogen free grade male SD rats were randomly divided into normal group(group N),model group(group M),phenytoin sodium group(group P)and RAGE receptor blocker group(group R),with 10 rats in each group.The epilepsy model was established by intraperitoneal injection of pentylenetetrazol in group M,group P and group R,but not in group N.After successful modeling,20 mg/kg phenytoin sodium was injected intraperitoneally in P group,1 mg/kg RAGE receptor blocker FPS-ZM was injected intraperitoneally in group R,and the same volume of 0.9%sodium chloride solution was injected intraperitoneally in group N and group M.The behavior of the young rats in each group was observed,the EEG was recorded,the neuronal injury damage was detected by FJB staining and Nissl staining,and the expression of S100 B protein was detected by immunoenzymatic labeling.Results Compared with group N,the Racine score,seizure duration and seizure times of young rats in group M,group P,group R were significantly increased(P<0.05);compared with group M,the Racine score,seizure duration and seizure times of young rats in group P and group R were significantly decreased(P<0.05);and compared with group P,above index in the group R were significantly decreased(P<0.05).Compared with group N,the amplitude of wave in group M,group P,group R was significantly increased(P<0.05),and the frequency was significantly decreased(P<0.05);compared with group M,the amplitude of wave in group P and group R was significantly decreased(P<0.05),and the frequency was significantly increased(P<0.05);and compared with group P,group R was significantly changed(P<0.05).Compared with group M,the neuronal injury in group P and group R was significantly reduced(P<0.05),the number of neurons increased(P<0.05),the morphology of neurons in group P and group R was improved(P<0.05),the
作者
姜小花
陈利娟
陈敏
耿立建
JIANG Xiaohua;CHEN Lijuan;CHEN Min;GENG Lijian(Department of Pediatrics,Xuzhou Municipal Hospital Affiliated to Xuzhou Medical University,Xuzhou Jiangsu 221000,China)
出处
《中国医疗设备》
2023年第5期156-160,共5页
China Medical Devices
基金
徐州医科大学附属医院发展基金自助项目(XYFM2021039)。
