摘要
目的探讨丁香酚对异丙肾上腺素(ISO)诱导的急性心肌梗死(AMI)模型大鼠的保护作用及其机制。方法40只Wistar大鼠按随机数字表法分成对照组、模型组和丁香酚低、中、高剂量组,每组8只。连续2 d注射ISO建立AMI模型大鼠,不同剂量(1、10和100 mg/kg)丁香酚处理后,采用酶联免疫吸附试验(ELISA)测定肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)、乳酸脱氢酶(LDH)、心肌肌钙蛋白T(cTnT)、肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)、丙二醛(MDA)和超氧化物歧化酶(SOD)水平,2,3,5-氯化三苯基四氮唑(TTC)染色计算大鼠心脏梗死面积,流式细胞仪检测心肌细胞凋亡水平,Western blot分析iNOS和Cleaved Caspase 3蛋白表达。结果与对照组比较,模型组大鼠CK[(0.93±0.06)U/mL比(0.22±0.04)U/mL,P<0.01]、CK-MB[(210.06±11.96)IU/L比(81.61±3.36)IU/L,P<0.01]、LDH[(5870.87±681.37)U/L比(1699.72±250.51)U/L,P<0.01]、cTnT[(0.63±0.09)U/mL比(0.07±0.03)U/mL,P<0.01]、iNOS蛋白表达[(1.15±0.02)比(0.42±0.03),P<0.01]、TNF-α[(318.61±28.27)pg/mg比(86.68±6.24)pg/mg,P<0.01]、IL-6[(5.33±0.98)pg/mg比(1.76±0.30)pg/mg,P<0.01]、MDA[(7.38±0.54)nmol/mg比(2.14±0.43)nmol/mg,P<0.01]和Cleaved Caspase 3蛋白表达[(1.13±0.07)比(0.64±0.03),P<0.01]升高,SOD含量[(82.46±14.77)U/mg比(188.19±19.34)U/mg,P<0.01]下降,梗死面积[(45.95±3.35)%比(0.00±0.00)%,P<0.01]和心肌细胞凋亡[(41.74±5.02)%比(9.05±0.71)%,P<0.01]增加。中、高剂量丁香酚给药后,AMI大鼠CK[(0.64±0.02)U/mL、(0.45±0.06)U/mL比(0.93±0.06)U/mL,P<0.01]、CK-MB[(144.11±8.28)IU/L、(121.10±7.88)IU/L比(210.06±11.96)IU/L,P<0.01]、LDH[(4076.81±465.13)U/L、(3438.28±361.19)U/L比(5870.87±681.37)U/L,P<0.01]、cTnT[(0.40±0.03)U/mL、(0.26±0.04)U/mL比(0.63±0.09)U/mL,P<0.01]、iNOS蛋白表达[(0.62±0.03)、(0.41±0.07)比(1.15±0.02),P<0.01]、TNF-α[(252.79±26.56)pg/mg、(222.29±18.40)pg/mg比(318.61±28.27)pg/mg,P<0.01]、IL-6[(3.33±0.47)pg/mg、(2.91±0.52)pg/mg比(5.33±0.98)pg/mg,P<0
Aim To investigate the protective effect and mechanism of eugenol on acute myocardial infarction(AMI)caused by isoproterenol(ISO)in rats.Methods Forty Wistar rats were randomly divided into control group,model group,eugenol low,middle and high dose groups,8 rats in each group.The rat AMI model was established by continuous 2 d injection of ISO,and creatine kinase(CK),CK-MB Isoenzyme(CK-MB),lactate dehydrogenase(LDH),cardiac troponin T(cTnT),tumor necrosis factor-α(TNF-α),interleukin 6(IL-6),malondialdehyde(MDA)and superoxide dismutase(SOD)levels were measured by enzyme linked immunosorbent assay(ELISA)method.2,3,5-Triphenyltetrazolium chloride(TTC)staining was used to calculate rat heart infarct area,and flow cytometry was used to detect cardiomyocyte apoptosis.Besides,the protein expression levels of iNOS and Cleaved Caspase 3 were determined using Western blot.Results Compared with the control group,in the model group,CK(0.93±0.06 U/mL vs.0.22±0.04 U/mL,P<0.01),CK-MB(210.06±11.96 IU/L vs.81.61±3.36 IU/L,P<0.01),LDH(5870.87±681.37 U/L vs.1699.72±250.51 U/L,P<0.01),cTnT(0.63±0.09 U/mL vs.0.07±0.03 U/mL,P<0.01),iNOS protein expression(1.15±0.02 vs.0.42±0.03,P<0.01),TNF-α(318.61±28.27 pg/mg vs.86.68±6.24 pg/mg,P<0.01),IL-6(5.33±0.98 pg/mg vs.1.76±0.30 pg/mg,P<0.01),MDA(7.38±0.54 nmol/mg vs.2.14±0.43 nmol/mg,P<0.01),and Cleaved Caspase 3 protein expression(1.13±0.07 vs.0.64±0.03,P<0.01)were increased,while SOD content(82.46±14.77 U/mg vs.188.19±19.34 U/mg,P<0.01)decreased,the infarct area(45.95±3.35%vs.0.00±0.00%,P<0.01),and myocardial cell apoptosis(41.74±5.02%vs.9.05±0.71%,P<0.01)were increased.After administration of eugenol,the levels of CK(0.64±0.02 U/mL,0.45±0.06 U/mL vs.0.93±0.06 U/mL,P<0.01),CK-MB(181.75±9.09 IU/L,144.11±8.28 IU/L,121.10±7.88 IU/L vs.210.06±11.96 IU/L,P<0.01),LDH(4841.55±265.25 U/L,4076.81±465.13 U/L,3438.28±361.19 U/L vs.5870.87±681.37 U/L,P<0.05),cTnT(0.40±0.03 U/mL,0.26±0.04 U/mL vs.0.63±0.09 U/mL,P<0.01),iNOS(0.88±0.02,0.62±0.03,0.41±0.07 vs.1.
作者
黄潇潇
黄连军
HUANG Xiaoxiao;HUANG Lian-jun(Department of Cardiology,First People's Hospital of Linhai City,Linhai,Zhejiang 317000,China)
出处
《浙江中西医结合杂志》
2023年第5期406-411,共6页
Zhejiang Journal of Integrated Traditional Chinese and Western Medicine
