摘要
目的 SD大鼠心肌缺血再灌注损伤(MIRI)模型建立需对大鼠进行缺血及再灌注,创伤大,耗时长,极易导致大鼠死亡。文中以高效、快速的方法建立大鼠MIRI模型,并确保造模动物具有较高的成功率及存活率。方法取80只成年SD大鼠(8~10周龄;体重控制在250~300 mg),给予戊巴比妥钠溶液50 mg/kg腹腔麻醉。在经气管插管接呼吸机辅助呼吸条件下,离断第2-3肋间肌肉充分暴露心脏,迅速定位结扎左冠状动脉前降支动脉1 h,松开结扎线后48 h建立心肌缺血再灌注模型。同时在术前、术中及术后1 h及48 h行心电图检查。术后48 h在相同部位再次结扎,行Evans-TTC双染,以显示心肌缺血及梗死面积大小。结果造模总存活率为90%,IR模型总成功率86.25%。LAD结扎后出现T波高耸、ST段抬高,与QRS波融合形成"墓碑样"改变,然后出现ST段逐渐回落、R波电压逐渐降低,可见左室前壁结扎线以下心肌及心尖部心肌颜色变为灰白色或者暗灰色,且持续存在;心脏搏动频率较结扎前明显下降,且搏动幅度较前明显减弱;术后48 h可见明显的苍白梗死区,与周围组织分界明显,通过切片及Evans Blue-TTC双染后可见层次明显的梗死区、缺血及正常心肌。每只造模成功SD大鼠缺血区面积+梗死区面积与左室总心肌组织面积比值0.43~0.55(P>0.05);梗死区面积与缺血区+梗死区面积比值0.36~0.45(P>0.05)。结论可以高效、快速地建立心肌缺血再灌注模型,具有降低模型动物死亡率及提高模型建立的成功率等特点。
Objective The establishment of the model of myocardial ischemia-reperfusion injury （MIRI） in SD rats involves lengthy time, severe damage, and death of the rats. We aimed at an efficient and rapid method for establishing an MIRI model in rats to ensure a high success rate of modeling and survival of the animals. Methods Using the trachea intubation-assisted breathing ma- chine, we fully exposed the hearts of 80 adult SD rats （8-10 weeks old and weighing 250-300 mg） by mutilating the 2-3 intercostal muscles. Then, we rapidly positioned and ligated the left anterior descending （LAD） coronary artery for an hour, and established the model of MIRI at 48 hours after releasing the slipknot. We performed electrocardiography （ECG） before, during, and at 1 and 48 hours after operation, ligated the same Dart again at 48 hours postoperatively, and measured the size of the myocardial ischemia and infarction areas using Evans-TTC doable staining. Results The survival rate of the 80 rats was 90% and the success rate of MIRI modeling was 86.25%. After ligation of the LAD coronary artery, ECG showed the ST segment and T wave elevation, followed by gradual decrease of the ST segment and R wave voltage, the myocardium and cardiac apex muscle grey or dark grey below the ligation slipknot. The heart rate myocardial motion amplitude were obviously reduced after ligation.Evans-TYC double staining revealed an evident myocardial infarction area at 48 hours after modeling and the ratio of the ischemia area ＋ infarction area to the total myocardial area of the left ventricle was 0.43 to 0.55 （P〉0.05） and that of the infarction area to the ische- mia area ＋ infarction area was 0.35 to 0.45 （P〉0.05）. Conclusion The modified rat model of MIRI can be fast and efficiently es- tablished, with a high success rate of modeling and a low mortality of the animals.
出处
《医学研究生学报》
CAS
北大核心
2017年第4期413-417,共5页
Journal of Medical Postgraduates
关键词
气管插管
缺血再灌注
动物模型
SD大鼠
Trachea cannula
Ischemia reperfusion
Animal model
Sprague-Dawley rats
