摘要
目的建立辽东楤木Aralia elata叶指纹图谱,并寻找能够反映辽东楤木叶抗肿瘤活性能力的质量评价方法。方法采用70%乙醇和水饱和的正丁醇提取各批次辽东楤木叶总皂苷,运用HPLC-ELSD测试分析皂苷并建立指纹图谱,MTT法测试楤木叶总皂苷和单体皂苷对人肺腺癌A549细胞、人肝癌SMMC-7221细胞、人结肠癌HT-29细胞的抑制作用,用灰色关联法分析指纹图谱峰与抗肿瘤作用之间的关系。结果建立了含有28个共有峰的辽东楤木叶HPLC-ELSD指纹图谱,各批次图谱与共有模式图谱间相似度在0.850以上,指认并归属其中5个峰成分。各批次楤木叶总皂苷抗肿瘤作用的半数抑制浓度(half inhibitory concentration,IC50)为7.39~79.13 mg/L,其中,黑龙江凤凰山7月的样品抗肿瘤效果最佳。谱-效相关性表明28个共有峰成分与抗肿瘤活性的关联度为0.4692~0.8103,其中10个成分与抗肿瘤活性间的关联度大于0.70,8个成分与抗肿瘤活性间的关联度小于0.60。图谱中有2个峰成分在各批次药材中含量都相当大,但谱-效关联度却很小。5个已知的峰成分中,楤木皂苷TTP和楤木皂苷Ⅴ抗肿瘤作用的IC50均小于190 mg/L,楤木皂苷Ⅳ、Ⅵ、Ⅶ抗肿瘤作用的IC50均大于200 mg/L。结论辽东楤木叶HPLC指纹图谱及抗肿瘤相关性分析可以为辽东楤木叶的质量评价提供参考。若从已知的5种楤木皂苷中挑选质量控制成分,楤木皂苷TTP和Ⅴ要优于楤木皂苷Ⅳ、Ⅵ、Ⅶ。
Objective To establish the fingerprint of Aralia elata leaves and find a quality evaluation method that can reflect the anti-tumor activity of Aralia elata leaves.Methods Total saponins were extracted from A.elata leaves in each batch with 70%ethanol and n-butanol.HPLC-ELSD was used to analyze saponins and establish fingerprint combined with the similarity analysis.MTT method was used to test the inhibitory effect of total saponins and monomer saponins of A.elata leaves on A549,SMMC-7221 and HT-29 cells.The relationship between the fingerprints and antitumor effect was analyzed by gray correlation analysis.Results The HPLC-ELSD fingerprint of A.elata leaves containing 28 common peaks was established.The similarity between each batch map and common pattern map was above 0.850,and five peak components were identified and assigned.The half inhibitory concentration(IC50)of each batch of total saponins in anti-tumor effect was(7.39-79.13)mg/L,among which,the samples from Fenghuang mountain in Heilongjiang Province had good anti-tumor effect,and the samples from July had good anti-tumor effect.The results of correlation between spectrum and effect showed that the correlation degree between 28 common peak components and anti-tumor activity was 0.4692-0.8103.The correlation coefficient between 10 peaks and anti-tumor activity was greater than 0.70,and the correlation degree between eight peaks and anti-tumor activity was less than 0.60.There were two peaks in the spectrum,which were quite large in each batch of medicinal materials,but the spectrum effect correlation was very small.Among the five known saponins,IC50 of A.elata saponin TTP and A.elata V in anti-tumor effect were less than 190 mg/L,IC50 of A.elata saponinⅣ,ⅥandⅦin anti-tumor effect were greater than 200 mg/L.Conclusion HPLC fingerprint of A.elata leaves and correlation analysis of anti-tumor can provide reference for quality evaluation of A.elata leaves.If the quality control components are selected from the five known aralia saponins,A.elata saponins
作者
邹淑君
贾力维
吕邵娃
管庆霞
张又天
李靖
许树军
ZOU Shu-jun;JIA Li-wei;LYU Shao-wa;GUAN Qing-xia;ZHANG You-tian;LI Jing;XU Shu-jun(School of Pharmacy,Heilongjiang University of Chinese Medicine,Harbin 150040,China;Laboratory Training Center,Heilongjiang University of Chinese Medicine,Harbin 150040,China)
出处
《中草药》
CAS
CSCD
北大核心
2023年第7期2172-2181,共10页
Chinese Traditional and Herbal Drugs
基金
黑龙江省中医药管理局项目(ZHY18-076)
中国博士后科学基金项目(2014M551289)
黑龙江中医药大学药学院本科科研专项(2019BK09)
黑龙江中医药大学优秀青年骨干教师项目(2020GG26)
黑龙江省中医药管理局项目(ZHY2020-078)。
关键词
辽东楤木叶
HPLC指纹图谱
抗肿瘤作用
灰关联分析
楤木皂苷Ⅳ
楤木皂苷Ⅴ
楤木皂苷Ⅵ
楤木皂苷Ⅶ
楤木皂苷TTP
Aralia elata(Miq.)Seem.leaves
HPLC fingerprint
anti-tumor activity
grey relational analysis
Aralia elata saponin IV
Aralia elata saponin V
Aralia elata saponin VI
Aralia elata saponinⅦ
Aralia elata saponin TTP
