摘要
目的观察枸杞多糖对去势C57BL/6J小鼠小脑的神经保护和认知功能改善作用。方法选用12周龄C57BL/6J雌鼠,随机分为假手术组、模型组和枸杞多糖(LBP)干预组。12周龄雌鼠去势后1周,LBP干预组给予灌胃枸杞多糖0.1 g·kg^(-1),每天1次,连续12周,假手术组和模型组灌胃服用等量蒸馏水。应用旷场实验、新物体识别实验、Morris水迷宫实验检测小鼠焦虑及学习记忆能力;酶联免疫吸附(ELISA)法检测小鼠血清中雌二醇(E2)含量;生物化学方法检测血清及小脑半球氧化应激指标丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)含量;蛋白免疫印迹法检测小脑半球胆碱乙酰转移酶(ChAT)及炎性因子白细胞介素(IL)-1β、IL-6、肿瘤坏死因子-α(TNF-α)的相对表达量。结果去势小鼠LBP干预12周后,与假手术组小鼠比较,模型组小鼠的体质量增加、子宫系数下降、学习记忆能力减弱、血清E2含量下降、血清及小脑半球氧化应激指标MDA含量增高,SOD和GSH-Px活力降低,小脑半球ChAT的表达量下调以及炎性因子IL-1β、IL-6、TNF-α的表达量上调(P均<0.05)。与模型组小鼠比较,LBP干预组小鼠的体质量、子宫系数及血清E2含量差异无统计学意义(P均>0.05),学习记忆能力、氧化应激指标MDA含量,SOD和GSH-Px活力、小脑半球ChAT及炎性因子IL-1β、IL-6、TNF-α的相对表达量均有所改善(P均<0.05)。结论LBP干预可以改善去势C57BL/6J小鼠的认知功能,可能与其对小脑的抗氧化、抗炎及提高ChAT的表达有关。
Objective To observe the neuroprotective and cognitive improvement effects of Lycium barbarum polysaccharides on cerebellum of ovariectomized C57BL/6J mice.Methods 12-week-old C57BL/6J female mice were randomly divided into sham group,model group and LBP group.One week after the 12-week-old female mice were castrated,the LBP group was intragastrically administered with LBP 0.1 g·kg^(-1)once a day for 12 weeks,the sham group and the model group were intragastrically administered with the same amount of distilled water.Open field test,new object recognition test and Morris water maze test were used to detect anxiety and learning and memory ability of mice.The content of estradiol(E2)in serum of mice was detected by ELISA.The contents of MDA,SOD and GSH-Px in serum and cerebellar hemisphere were detected by biochemical method.The relative expression of ChAT and inflammatory factors IL-1β,IL-6,TNF-αin cerebellar hemisphere were detected by Western blot.Results After 12 weeks of LBP intervention in ovariectomized mice,compared with the sham group,the body weight of the mice in the model group increased,the uterine coefficient decreased,the learning and memory ability decreased,the serum E2 content decreased,the serum and cerebellar hemisphere oxidative stress index MDA content increased,the SOD and GSH-Px activity decreased.The expression of ChAT in the cerebellar hemisphere was down-regulated and the expression of inflammatory factors IL-1β,IL-6 and TNF-αwas up-regulated(P all<0.05).Compared with the model group,there was no significant difference in body weight,uterine coefficient and serum E2 content in LBP group(P all>0.05),while the learning and memory ability and oxidative stress index MDA content,the activities of SOD and GSH-Px,the relative expression of ChAT and inflammatory cytokines IL-1β,IL-6,TNF-αin cerebellum were improved(P all<0.05).Conclusion LBP can improve the cognitive function of ovariectomized C57BL/6J mice,which may be related to its anti-oxidation,anti-inflammation and up-regulation of C
作者
王晓爽
刘艺洁
丁雪
李林隆
李海林
赵薇
王银
丁玉梅
苗珍花
WANG Xiaoshuang;LIU Yijie;DING Xue;LI Linlong;LI Hailin;ZHAO Wei;WANG Yin;DING Yumei;MIAO Zhenhua(Ningxia Medical University,Yinchuan 750004,China;Department of Traditional Chinese Medicine,the General Hospital of Ningxia Medical University,Yinchuan 750004,China)
出处
《宁夏医科大学学报》
2023年第4期348-355,共8页
Journal of Ningxia Medical University
基金
国家自然科学基金项目(81060034,81460220)
宁夏回族自治区重点研发项目(2021BEG03097)
宁夏医科大学大学生创新创业训练计划项目(X202210752009)。
关键词
枸杞多糖
去势
C57BL/6J小鼠
小脑
神经保护
lycium barbarum polysaccharides
ovariectomized
C57BL/6J mice
cerebellum
neuroprotection
