摘要
【目的】探讨取卵日不同径线卵泡的黄素化颗粒细胞中各种水通道蛋白(AQPs)的mRNA表达水平。【方法】收集2022年3月25日至2022年9月23日在本中心行体外受精胚胎移植助孕的不孕患者取卵日的卵泡液共48例,根据卵泡径线分为小(<13 mm)、中(13~18 mm)、大(≥18 mm)卵泡群分开收集卵泡液提取颗粒细胞。经RNA定量后纳入22例进行分析,比较3组卵泡群的AQPs mRNA表达水平。根据促排卵方案将患者分为拮抗剂组和激动剂组,分析AQPs的表达差异。【结果】黄素化颗粒细胞中AQP2的mRNA水平随卵泡径线的增加而升高(linear trend P=0.004),其差异在大、小卵泡组间具有统计学差异(P=0.017);该差异在拮抗剂组内具有统计学意义(P=0.0496),在激动剂组中无统计学意义(P=0.108)。【结论】AQP2在黄素化颗粒细胞中的mRNA水平随着卵泡径线的增长而增加,其表达量与促排卵方案有关,提示AQP2在卵泡生长和卵泡液生成过程中可能起到一定作用,且其mRNA表达水平可能受到FSH和LH的调节。
【Objective】To investigate the mRNA expression levels of various aquaporins(AQPs)in luteinized granulosa cells from follicles of different diameters.【Methods】From March 25,2022 to September 23,2022 in our reproductive medicine center,48 women undergoing in-vitro fertilization(IVF)were enrolled and divided into the antagonist group and the agonist group according to the ovarian stimulation protocol.Follicular fluid samples were collected on the day of oocyte pick-up and granulosa cells were extracted from follicles of different diameters:small(<13 mm),medium(13~18 mm)and large(≥18 mm).After RNA quantification,22 cases(66 samples)were included for analysis and mRNA expression levels of AQPs were compared among the three follicle groups.【Results】The mRNA expression of aquaporin 2(AQP2)in luteinized granulosa cells increased with the increase of follicle diameter(linear trend P=0.004)and the difference was statistically significant between two groups of large and small follicles(P=0.017).Statistical difference was found in the antagonist group(P=0.0496),but not in the agonist group(P=0.108).【Conclusion】The mRNA level of AQP2 in luteinized granulosa cells increases with the increase of follicle diameter and its expression is related to the ovarian stimulation protocol,suggesting that AQP2 may play a role in follicle growth and follicular fluid formation,and its mRNA expression level may be regulated by follicle stimulating hormone(FSH)and luteinizing hormone(LH).
作者
郑泽桐
谈际范
李宇彬
张秀冰
刘思敏
周灿权
ZHENG Ze-tong;TAN Ji-fan;LI Yu-bin;ZHANG Xiu-bing;LIU Si-min;ZHOU Can-quan(Reproductive Center,The First Affiliated Hospital,Sun Yat-sen University/Guangdong Key Laboratory of Reproductive Medicine,Guangzhou 510080,China)
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2023年第3期462-470,共9页
Journal of Sun Yat-Sen University:Medical Sciences
基金
广东省生殖医学重点实验室(2020B1212060029)
广东省自然科学基金(2021A1515010559)。
关键词
水通道蛋白
黄素化颗粒细胞
卵泡液
卵泡生长
aquaporins(AQPs)
luteinized granulosa cells
follicular fluid
follicle growth
