摘要
目的:探讨控制性超促排卵(COH)周期多囊卵巢综合征(PCOS)患者卵巢颗粒细胞生长分化因子-9(GDF-9)、骨形成蛋白-15(BMP-15)的表达及其与体外受精-胚胎移植(IVF-ET)治疗结局的关系。方法:选择接受IVF-ET治疗的PCOS患者45例,同期输卵管因素不孕患者103例作为对照组。应用免疫组织化学、Real-time PCR技术检测患者卵巢颗粒细胞GDF-9、BMP-15蛋白和mRNA的表达,并分析与IVF-ET的临床结局关系。结果:GDF-9、BMP-15蛋白在PCOS组和对照组患者卵巢颗粒细胞胞质均呈阳性表达,PCOS患者颗粒细胞GDF-9 mRNA、BMP-15 mRNA的表达水平低于对照组,差异有统计学意义(P<0.01)。多元线性回归分析显示,颗粒细胞GDF-9 mRNA、BMP-15 mRNA表达水平与优质胚胎数呈正相关关系(P<0.05)。临床妊娠组的颗粒细胞GDF-9mRNA、BMP-15 mRNA表达水平高于未妊娠组(P<0.05)。结论:COH周期PCOS患者颗粒细胞GDF-9 mRNA、BMP-15 mRNA表达水平可能与胚胎发育潜能存在相关性。
Objective： To explore the expression levels of growth differentiation factor 9 （GDF-9） and bone morphogenetic protein 15 （BMP-15） in granulosa cells of patients with polycystic ovary syndrome （PCOS） during controlled ovarian hyperstimulation （COH） cycles and the relationship between the expression levels of above two genes and the outcomes of in vitro fertilization and embryo transfer （IVF-ET）. Methods： Totally 43 PCOS patients and 103 infertile cases due to tubal factors who underwent IVF-ET at the same period were recruited as the experimental group and control group, respectively. Immunocytochemistry and Real-time PCR were used to detect the expression levels of GDF-9, BMP-15 proteins and their mRNA in granulosa cells of the two groups, respectively. And their relationship with the outcomes of IVF-ET was analyzed. Results： Both GDF-9 and BMP-15 proteins were detected in the cytoplasm of granulosa cells in the two groups. The expression levels of GDF-9, BMP-15 mP, NA in granulosa cells in PCO S group were significantly lower than those in control group （P〈0.01）. Multivariate linear analysis discovered that GDF-9, BMP-15 rnRNA levels were positively associated with the number of high-quality embryos （P〈0.05）. The expression levels of GDF-9 and BMP-15 mRNA in pregnancy group were significantly higher than those in non-pregnancy group （P〈0.05）. Conclusion： GDF-9, BMP-15 mRNA levels in granulosa cells of PCOS patients during COH cycles may be associated with embryo developmental potential.
出处
《生殖与避孕》
CAS
CSCD
北大核心
2016年第4期276-283,共8页
Reproduction and Contraception
基金
福建省自然科学基金计划面上项目(2013J01267)
福建省临床重点专科建设项目(闽卫科教2012149)
福建卫生系统中青年骨干人才培养项目(2013-ZQN-JC-7)
