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农耕酵素堆肥进程中微生物菌群动态变化研究

Study on Dynamic Changes of Microbial Community during Agricultural Composting
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摘要 农耕酵素堆肥是解决农业面源污染的最佳途径,微生物数量及多样性决定堆肥的品质及腐熟速度。本项目主要研究在农耕酵素堆肥进程中,添加不同发酵菌剂,观测堆肥中细菌、霉菌、放线菌等主要微生物,伴随温度、pH、电导率等诸多因素变化,各大类微生物的数量及各菌群丰富度变化规律。结果表明:发酵菌剂添加能增加主发酵期堆肥菌体的数量,能加速升温腐熟进程;细菌、放线菌数量变化趋势是增加-减少-增加,而霉菌是增加-减少,菌落丰富度呈现先下降后又上升的趋势;主发酵期处理组均高于空白对照组,从效率、经济方面综合看,BCD组优于BCL组和BCZ组,但到腐熟后期,BC组和CK组差异不显著,G-致病菌被灭杀。 Agricultural enzymes compost is the best mean for solving agricultural non-point source pollution and the number and diversity of microorganisms determine the quality and speed of decomposition of compost.This project mainly studies the addition of different fermenting agents in the process of agricultural enzyme composting,and observes the main microorganisms such as bacteria,mold and actinomycetes in the compost,as well as the changes in the number of microorganisms and the richness of each flora with the changes of temperature,pH,EC and other factors.The results showed that the addition of fermentation agent can increase the number of compost bacteria in the main fermentation stage and accelerate the process of heating and rotting.The change of the number of bacteria and actinomycetes follows an increase-decrease-increase trend,while the number of mold increases first and then decreases.The colony richness follows a trend of decrease-increase.The BC group in the main fermentation stage is higher than the CK group,and from the comprehensive perspective of efficiency and economy,the BCD group is better than the BCL group and the BCZ group.However,in the late stage of rotting,the difference between the BC group and the CK group was seen non-significant,and the G-pathogenic bacteria were killed.
作者 张丽君 邓恵静 杨乾坤 杨正芳 ZHANG Li-jun;DENG Hui-jing;YANG Qian-kun;YANG Zheng-fang(School of Biomedical and Chemical Engineering,Liaoning Institute of Science and Technology,Benxi Liaoning 117004,China)
出处 《辽宁科技学院学报》 2023年第2期8-11,34,共5页 Journal of Liaoning Institute of Science and Technology
基金 2021年辽宁科技学院校级大创项目“农耕酵素堆肥进程中微生物菌群动态变化研究”(202111430055).
关键词 农耕酵素 堆肥 发酵剂 微生物菌群 Agricultural enzyme Compost Starter cultures Microbial flora
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