摘要
为探究miR-19a对民猪脂肪细胞分化过程的调控机制,研究采用双荧光素酶报告载体、实时荧光定量PCR构建组织表达谱、过表达和抑制miR-19a后对成脂标志基因和脂肪酸代谢标志基因的影响,油红O染色等生物学技术分析miR-19a对前脂肪细胞成脂的影响。结果表明,miR-19a与KLF13呈靶向关系,过表达miR-19a抑制成脂标志基因表达,促进脂肪酸代谢标志基因表达,抑制脂滴形成。抑制miR-19a可促进成脂标志基因表达,抑制脂肪酸代谢标志基因表达,促进脂滴形成。综上,试验验证miR-19a可抑制前脂肪细胞成脂分化,确定KLF13为miR-19a靶基因,为进一步研究microRNA对民猪前脂肪细胞分化的调控机制提供参考。
The aim was to investigate the effect of miR-19a on the differentiation process of adipocytes in Min pigs and to provide a basis for further researches on the molecular mechanism of adipocyte differentiation in Min pigs.The effects of miR-19a on preadipocyte lipogenesis were analyzed by biological techniques such as dual luciferase reporter vector,real-time fluorescence quantitative PCR to construct tissue expression profiles,detection of the effects on lipogenic marker genes and fatty acid metabolism marker genes after overexpression and inhibition of miR-19a,and oil red O staining.The results showed that miR-19a and KLF13 were targeting relationship.Overexpression of miR-19a inhibited lipogenesis marker gene expression,promoted fatty acid metabolism marker gene expression and inhibited lipid droplet formation;inhibition of miR-19a promoted lipogenesis marker gene expression,inhibited fatty acid metabolism marker gene expression and promoted lipid droplet formation.In summary,this experiment verified that miR-19a could inhibit preadipocyte lipogenic differentiation and identified KLF13 as the target gene of miR-19a.It also provided a reference for further study on the regulation of microRNA on preadipocyte differentiation in civilian pigs.
作者
刘娣
李皓月
马红
汪亮
LIU Di;LI Haoyue;MA Hong;WANG Liang(School of Animal Sciences and Technology,Northeast Agricultural University,Harbin 150030,China;Institute of Animal Husbandry,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China)
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2023年第4期64-72,共9页
Journal of Northeast Agricultural University
基金
国家自然科学基金项目(U20A2052)
黑龙江省省属科研院所科研业务费项目(CZKYF2021-2-C025)
国家生猪技术创新中心项目(NCTIP-XD1C16)。
