摘要
目的基于UHPLC-MS/MS建立同时测定白芷中34种植物生长调节剂残留量的分析方法。方法样品通过乙腈高速匀浆提取,浓缩后直接进样,采用Waters Acquity UPLC HSS T3(2.1 mm×150 mm,1.8μm)色谱柱,流动相0.1%甲酸水溶液含10 mmol·L^(–1)甲酸铵(A)-乙腈(B),用基质匹配标液外标法进行定量。结果34种植物生长调节剂在基质中的线性关系良好,相关系数均>0.995,检出限为0.01~9.26μg·kg^(-1),平均回收率为72.4%~100.6%,RSD均<15%。采用该法对35批白芷样品中的植物生长调节剂残留情况进行筛查,其中19批样品中检测出6种植物生长调节剂。结论建立的植物生长调节剂多残留检测方法灵敏度高,准确性好,有针对性,且前处理简单快速,可用于筛查和检测白芷中植物生长调节剂多残留量。
OBJECTIVE To establish an analytical method for the simultaneous determination of 34 plant growth regulators residues in Angelicae Dahuricae Radix based on UHPLC-MS/MS.METHODS The samples were extracted by acetonitrile high-speed homogenization,concentrated,injected directly without purification method,separated by a Waters Acquity UPLC HSS T3(2.1 mm×150 mm,1.8μm)column with mobile phase of 0.1%formic acid aqueous solution containing 10 mmol·L^(-1)ammonium formate(A)-acetonitrile(B).The matrix-matched external standard method was used for quantitative analysis.RESULTS The calibration curves of 34 plant growth regulators showed good linearity with correlation coefficients>0.995.The limits of detection were 0.01−9.26μg·kg^(-1),and average recoveries of 72.4%−100.6%,the RSD<15%.The plant growth regulators in 35 batches of Angelicae Dahuricae Radix were analyzed by this method.CONCLUSION The established method is sensitive,accurate,targeted,and simple with rapid pre-treatment,and can be used for screening and detection of plant growth regulator multi-residues in Angelicae Dahuricae Radix.
作者
褚悟男
李翔
韩玲玲
蒲婧哲
翟宏焱
王浩
张亚中
CHU Wunan;LI Xiang;HAN Lingling;PU Jingzhe;ZHAI Hongyan;WANG Hao;ZHANG Yazhong(School of Pharmacy,Anhui University of Traditional Chinese Medicine,Hefei 230012,China;Anhui Institute of Food and Drug Control,Hefei 230051,China;NMPA Key Laboratory for Quality Research and Evaluation of Traditional Chinese Medicine,Hefei 230051,China;Anhui Provincial Key Laboratory of New Manufacturing Technology for Traditional Chinese Medicine Decoction Pieces,Hefei 230051,China)
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2023年第6期783-791,共9页
Chinese Journal of Modern Applied Pharmacy
基金
国家药典委员会药品标准制修订课题(2021Z04)。
