摘要
目的:研究外源性脱落酸(ABA)对玄参幼苗耐盐碱能力的影响,为在盐碱地种植玄参提供理论依据。方法:50株玄参幼苗分为对照组、盐碱组(75 mmol/L)和盐碱(75 mmol/L)+ABA低、中、高浓度组(10、50、100μmol/L),每组10株。移栽20 d起,每4 d干预1次,2周后测定各组植株生长指标(茎高、鲜质量、干质量、存活率)、生理指标[叶绿素、可溶性糖(SS)、可溶性蛋白(SP)和游离脯氨酸(Pro)含量]、丙二醛(MDA)和过氧化氢(H_2O_2)含量、抗氧化酶活性[超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、谷胱甘肽还原酶(GR)活性]、茎和根中Na^+和K^+含量。结果:与对照组比较,盐碱组玄参幼苗茎高、存活率、SP、茎中Na^+和K^+、根中Na^+含量和CAT、GR活性显著降低,叶绿素、Pro、MDA、H_2O_2、根中K^+含量和SOD、POD活性显著升高(P<0.05或P<0.01)。与盐碱组比较,盐碱+ABA中浓度组玄参幼苗茎高、鲜质量、叶绿素含量显著增加,MDA、茎中Na^+含量显著降低;低、中浓度组干质量、SS、SP含量和SOD、CAT活性,高浓度组SS含量显著增加;高浓度组茎高、干质量显著降低;低、高浓度组Pro含量显著降低;低、中、高浓度组H_2O_2、根中Na+含量显著降低,GR活性、茎和根中K^+含量显著升高;中、高浓度组POD活性显著降低。结论:施加一定浓度的外源性ABA可有效提高玄参幼苗耐盐碱的能力,增强植株对盐碱环境的适应能力。
OBJECTIVE： To study the effects of exogenous abscisic acid （ABA） on salt-alkali tolerance of Scrophularia ning- poensis seedlings, and to provide theoretic evidence for cultivating S. ningpoensis in salt-alkali soil. METHODS： 50 S. ningpoensis seedlings were divided into control group, salt-alkali group （75 mmol/L）, salt-alkali （75 mmol/L）＋ABA low-concentration, medi- um-concentration and high-concentration groups （10, 50, 100 μmol/L）, with 10 plants in each group. 20 days after transplanting, the plants were sprayed with drugs every 4 days. Growth indexes of the plants （length of stem, fresh weight, dry weight, survival rate）, physiological indexes [the contents of chlorophyll, soluble sugar （SS）, soluble protein （SP） and free proline （Pro）], the contents of MDA and H2O2, the activities of antioxidase [the activities of SOD, POD, CAT, GR] and the contents of Na^＋ and K^＋ in stem and root were determined in each group 2 weeks later. RESULTS： Compared with control group, stem height and survival rate of S. ningpoensis seedlings, SP, the contents of Na^＋ and K^＋ in the stem, the contents of Na^＋, CAT and GR in the roots were all decreased significantly in salt-alkali group; while chlorophyll, Pro, MDA and H2O2 contents, the content of K^＋ in the roots and the activities of SOD and POD were all increased significantly （P〈0.05 or P〈0.01）. Compared with salt-alkali group, stem height of S. ningpoensis seedlings, fresh weight and chlorophyll content were increased significantly in salt-alkali＋ABA medium-concentra- tion group, while MDA and the content of Na^＋ in the stem were decreased significantly; dry weight, the contents of SS and SP, the activities of SOD and CAT were increased significantly in salt-alkali＋ABA low-concentration and medium-concentration groups, while the SS content was increased in salt-alkali＋ABA high-concentration group; stem height and dry weight were decreased signifi- cantly in salt-alkali ＋ ABA high-concentration group; Pr
作者
王建安
付英杰
王荣华
李艳芝
王欣
WANG Jian＇an FU Yingjie WANG Ronghua LI Yanzhi WANG Xin(College of Pharmaceutical Science, Jining Medical University, Shandong Rizhao 276826, China Rizhao Polytechnic, Shandong Rizhao 276826, China)
出处
《中国药房》
CAS
北大核心
2017年第4期573-576,共4页
China Pharmacy
基金
山东省医药卫生科技发展计划项目(No2013WS0333)
济宁医学院重点课题(No.JYZ013KJ007)
关键词
玄参
盐碱胁迫
外源性脱落酸
生长指标
抗氧化酶
ScrophuIaria ningpoensis
Salt-alkali stress
Exogenous abscisic acid
Growth indexes
Antioxidas
