摘要
文章通过对肉苁蓉中多糖物质进行提取,采用DEAE纤维素柱层析法对肉苁蓉粗多糖CLP进行纯化,获取肉苁蓉中性糖CLP1和肉苁蓉酸性糖CLP2。采用紫外光谱扫描的方法测定肉苁蓉粗多糖CLP、肉苁蓉中性糖CLP1和肉苁蓉酸性糖CLP2中的总糖、糖醛酸以及蛋白质含量,并对多糖分子量和单糖组成成分进行测定,同时对肉苁蓉粗多糖CLP、肉苁蓉中性糖CLP1和肉苁蓉酸性糖CLP2的DPPH自由基清除能力和总抗氧化能力进行实验分析。测定数据表明,肉苁蓉粗多糖CLP、肉苁蓉中性糖CLP1和肉苁蓉酸性糖CLP2的组成成分接近,但含量存在较大的差异;肉苁蓉中性糖CLP1和肉苁蓉酸性糖CLP2在利用DEAE纤维素色谱柱进行分离纯化时能够获取到多个洗脱峰,分子量分布在0~10 000 Da之间,表明肉苁蓉多糖主要组成成分为小分子糖。肉苁蓉中性糖CLP1单糖测定结果表明,肉苁蓉中性糖CLP1中含有的单糖主要为甘露糖、半乳糖醛酸、葡萄糖、半乳糖以及阿拉伯糖;肉苁蓉酸性糖CLP2单糖测定结果表明,肉苁蓉酸性糖CLP2中含有的单糖主要为鼠李糖、半乳糖醛酸、葡萄糖以及半乳糖。肉苁蓉中多糖的DPPH自由基清除实验和总抗氧化能力实验结果表明,肉苁蓉中性糖CLP1的抗氧化能力高于肉苁蓉粗多糖CLP和肉苁蓉酸性糖CLP2。
In this paper,polysaccharides are extracted from Cistanche deserticola,Cistanche deserticola crude polysaccharide CLP is purified by DEAE cellulose column chromatography,and neutral sugar CLP1 and acidic sugar CLP2 of Cistanche deserticola are obtained.The content of total sugar,uronic acid and protein in Cistanche deserticola crude polysaccharide CLP,Cistanche deserticola neutral sugar CLP1 and Cistanche deserticola acidic sugar CLP2 is determined by UV spectral scanning,and the molecular weight and monosaccharide composition of polysaccharides are determined.At the same time,the DPPH radical scavenging capacity and total antioxidant capacity of Cistanche deserticola crude polysaccharide CLP,Cistanche deserticola neutral sugar CLP1 and Cistanche deserticola acidic sugar CLP2 are analyzed.The measured data show that the components of Cistanche deserticola crude polysaccharide CLP,Cistanche deserticola neutral sugar CLP1 and Cistanche deserticola acidic sugar CLP2 are similar,but there are great differences in their content.Multiple elution peaks can be obtained when Cistanche deserticola neutral sugar CLP1 and Cistanche deserticola acidic sugar CLP2 are separated and purified by DEAE cellulose chromatographic column,and the molecular weight distribution is 0~10000 Da,indicating that the main components of Cistanche deserticola polysaccharides are small molecular sugars.The monosaccharide determination results of Cistanche deserticola neutral sugar CLP1 show that the monosaccharides in Cistanche deserticola neutral sugar CLP1 are mainly mannose,galacturonic acid,glucose,galactose and arabinose.The monosaccharide determination results of Cistanche deserticola acidic sugar CLP2 show that the monosaccharides in Cistanche deserticola acidic sugar CLP2 are mainly rhamnose,galacturonic acid,glucose and galactose.The results of DPPH radical scavenging experiment and total antioxidant capacity experiment of polysaccharides from Cistanche deserticola show that the antioxidant capacity of Cistanche deserticola neutral sugar
作者
肖林霞
苏远
XIAO Lin-xia;SU Yuan(Huanghe University of Science and Technology,Zhengzhou 450006,China)
出处
《中国调味品》
CAS
北大核心
2023年第5期71-74,共4页
China Condiment
基金
河南省高校人文社会科学研究一般项目(2022-ZDJH-00399)。
关键词
肉苁蓉
多糖物质
成分分析
抗氧化活性
吸光度
Cistanche deserticola
polysaccharides
component analysis
antioxidant activity
absorbance
