摘要
目的 确定赤芍多糖的最佳提取条件。方法 采用苯酚-硫酸法显色,紫外可见分光光度法(UV)在490nm波长处测定当归多糖的吸光度,以赤芍多糖得率为指标,采用单因素和Box-Behnken响应面法优化实验条件。结果 当归多糖的最佳工艺参数为:料液比1∶20.84(g∶m L),回流时间为157min 5s、回流次数3次。结论 最佳提取条件下获得的平均赤芍多糖得率为8.070%,与理论值之间的误差为0.36%,说明将Box-Behnken法应用于水提赤芍多糖工艺的优化是可行的,可用于赤芍多糖的提取。
OBJECTIVE To determine the best extraction conditions of Paeonia lactiflora polysaccharide.METHODS The absorbance radix paeoniae rubra polysaccharide was measured at 490nm by phenol-sulfuric acid chromogenesis and UV spectrophotometry,and the experimental conditions were optimized by single factor and Box-Behnken response surface method using the yield of radix peaoniae rubra polysaccharide as the index.RESULTS The optimal process parameters for the extraction of radix paeoniae rubra polysaccharide were:material-liquid ratio 1∶20.84 g·mL-1,reflux time 157min 5s,and reflux times 3 times.CONCLUSION The average yield of radix paeoniae rubra polysaccharide obtained under the optimal extraction conditions was 8.070%,and the error between it and the theoretical value was 0.36%,indicating that it is feasible to apply the Box-Behnken method to optimize the process of aqueous extraction of radix paeoniae rubra polysaccharide,which can be used for the extraction of radix paeoniae rubra polysaccharide.
作者
王文姌
王韫格
张洪财
孟祥才
WANG Wen-ran;WANG Yun-ge;ZHANG Hong-cai;MENG Xiang-cai(First Affiliated Hospital,Heilongjiang University of Chinese Medicine,Harbin 150040,China;Institute of Traditional Chinese Medicine,Heilongjiang University of Chinese Medicine,Harbin 150040,China;GAP Center,Heilongjiang University of Chinese Medicine,Harbin 150040,China)
出处
《化学工程师》
CAS
2023年第4期88-92,31,共6页
Chemical Engineer
基金
国家自然科学青年基金项目(81803711)
黑龙江省自然科学青年基金项目(QC2018118)
黑龙江中医药大学“优秀创新人才青年教师支持计划”项目(2018RCQ09)。
关键词
赤芍多糖
提取工艺
响应曲面分析
Box-Behnken
radix paeoniae rubra polysaccharide
extraction process
surface response methodology
Box-Behnken
