摘要
目的:用纤维素酶提取蓝莓果渣多酚,以多酚得率为指标优化提取工艺。方法:选取纤维素酶添加量、酶解pH、料液比、酶解时间、酶解温度等5种因素分别利用单因素试验和响应面分析法,研究蓝莓果渣多酚最佳提取工艺。结果:当纤维素酶用量为6.8 mg/g,提取初始pH为4.8,料液比为1∶20,提取时间为6 h,温度为50℃时,蓝莓果渣中多酚的得率最高,达到8.1306 mg/g。结论:纤维素酶水解法提取蓝莓果渣多酚,通过调整酶添加量、酶解pH、料液比、酶解温度、酶解时间可以显著增加蓝莓果渣多酚得率。
Objective:Blueberry fruit residue polyphenol was extracted by cellulase,and the extraction process was optimized according to the polyphenols yield rate.Methods:The five index factors of cellulase addition,enzymolysis pH,material liquid ratio,enzymolysis time,and enzymolysis temperature are selected,respectively,using single factor test and response surface analysis to study the best extraction technology of blueberry pomace polyphenols.Results:When the cellulase dosage is 6.8 mg/g,the initial extraction pH is 4.8,the material-liquid ratio is 1∶20,the extraction time is 6 h,and the temperature is 50℃,and the yield of polyphenols in blueberry pomace is the highest,reaching 8.1306 mg/g.Conclusion:The cellulase hydrolysis method is used to extract blueberry pomace polyphenols,and the yield of blueberry pomace polyphenols can be significantly increased by adjusting the amount of enzyme,the pH of the enzyme hydrolysis,the ratio of material to liquid,the temperature of the enzyme hydrolysis,and the time of the enzyme hydrolysis.
作者
王大全
王俊颖
翟立公
时志军
曹双双
杨剑婷
WANG Daquan;WANG Junying;ZHAI Ligong;SHI Zhijun;CAO Shuangshuang;YANG Jianting(College of Food Engineering,Anhui Science and Technology University,Fengyang 233100,China;Anhui Fengyang Jianming Blueberry Agriculture Co.,Ltd.,Fengyang 233100,China)
出处
《安徽科技学院学报》
2023年第1期47-53,共7页
Journal of Anhui Science and Technology University
基金
安徽省重点研究与开发项目(202104f06020021)
安徽省果树产业技术体系果品加工与流通岗位专家项目(AHCYTX-10)。
关键词
蓝莓果渣
多酚
纤维素酶
响应面分析
Blueberry fruit residue
Polyphenols
Cellulase enzyme
Response surface analysis
