摘要
该文研究了ASP(刺五加多糖)的抗运动性疲劳与抑制氧化应激作用。将小鼠随机分为对照组及刺五加多糖低、中、高剂量组(50、100、200 mg/kg),连续干预28 d。结果发现,与对照组比较,刺五加多糖低、中、高剂量组小鼠负重游泳时间延长了13.79%、33.68%和48.89%,肌糖原、肝糖原含量增加,BUN(血尿素氮)、乳酸、血糖及血氨含量降低;与对照组比较,刺五加多糖低、中、高剂量组小鼠骨骼肌GSH-Px(谷胱甘肽过氧物酶)活性增加了29.82%、53.03%和59.32%,SOD(超氧化物歧化酶)活性增加了9.64%、15.23%和50.05%,MDA(丙二醛)含量降低了16.45%、36.76%和45.82%;与对照组比较,刺五加多糖中、高剂量组小鼠骨骼肌NOX2及低、中、高剂量组NOX4、Keap1基因表达下降,而刺五加多糖各剂量组Nrf2及HO-1基因表达增加,同时相关蛋白的表达水平具有一致的趋势。结果表明,刺五加多糖具有抗小鼠运动性疲劳作用,该作用与抑制NOX2、NOX4表达及调节Nrf2/ARE信号通路,进而抑制氧化应激作用有关。
The anti-exercise-fatigue and oxidative stress inhibitory effects of Acanthopanax senticosus polysaccharide(ASP)was investigated.Mice were randomly divided into the control group and the low-,medium-and high dose ASP groups(50,100 and 200 mg/kg),and the intervention lasted for 28 days.The results showed that compared with the control group,the weight-bearing swimming times of mice for the low-,medium-and high dose ASP groups were prolonged by 13.79%,33.68%and 48.89%,respectively,the contents of muscle glycogen and liver glycogen increased,and the contents of BUN,lactic acid,blood glucose and blood ammonia decreased;Compared with the control group,the activity of GSH-Px in the skeletal muscle for the low-,medium-and high dose ASP mice increased by 29.82%,53.03%and 59.32%,respectively,their SOD activities increased by 9.64%,15.23%and 50.05%,respectively,and their MDA contents decreased by 16.45%,36.76%and 45.82%,respectively;Compared with the control group,NOX2 gene expression in the skeletal muscle of the medium-and high dose ASP groups and the NOX4 and Keap1 gene expressions of the low-,medium-and high dose ASP groups decreased,whilst Nrf2 and HO-1 gene expressions of all the ASP groups increased,and the expression levels of related proteins showed a consistent trend.The results showed that ASP has an anti-exercise-fatigue effect in mice,which is related to the inhibition of NOX2 and NOX4 expressions and the regulation of Nrf2/ARE signaling pathway,thereby inhibiting oxidative stress.
作者
古鹏鑫
贾奇侠
王海峰
GU Pengxin;JIA Qixia;WANG Haifeng(Department of Physical Education,Chongqing Jiaotong University,Chongqing 400074,China;Department of Physical Education,Northwest A&F University,Yangling 712100,China)
出处
《现代食品科技》
CAS
北大核心
2023年第4期55-61,共7页
Modern Food Science and Technology
基金
陕西省哲学社会科学重大理论与现实问题研究项目(2021ND03823)。
