摘要
目的研究Gap27抑制缝隙连接功能对豚鼠阴茎海绵体平滑肌细胞凋亡的影响。方法将40只成年雄性豚鼠随机分为4组,每组10只。其中正常组(normal组)不作处理;假手术组(sham组)在阴茎根部注射生理盐水;腺相关病毒组(AAV组)在阴茎根部注射GJA1基因过表达腺相关病毒来过表达Cx43蛋白;Gap27组在阴茎根部注射Gap27抑制缝隙连接功能。注射2周后取阴茎海绵体组织进行检测,通过免疫荧光观察Cx43在阴茎海绵体中的表达,Western blotting检测Cx43、α-SMC、Caspase-3和Caspase-9蛋白的表达,Masson三色染色检测海绵体平滑肌含量。结果Cx43在阴茎海绵体大量表达,主要表达于细胞膜上。与normal组相比,AAV组和Gap27组α-SMC表达降低、Caspase-3和Caspase-9表达升高(P<0.05);Masson三色染色结果表明AAV组和Gap27组平滑肌含量降低。结论Gap27抑制细胞间缝隙连接功能会导致平滑肌细胞发生凋亡。
Objective To study the effect of Gap27 inhibition of gap junction on apoptosis of corpus cavernosum smooth muscle cells in guinea pigs.Methods 40 adult male guinea pigs were randomly divided into 4 groups,with 10 guinea pigs in each group.Normal group was not treated;sham operation group(sham group)was injected saline into penile root;adeno-associated virus group(AAV group)was injected GJA1 gene overexpression adeno-associated virus into penile root to overexpress connexin 43(Cx43)protein;and Gap27 group was injected Gap27 into penile root to inhibit gap junction function.Two weeks after injection,the corpus cavernosum was detected.The expression of Cx43 in the corpus cavernosum was observed by immunofluorescence,the expression of Cx43,α-SMC,Caspase-3 and Caspase-9 was detected by Western blotting,and the content of smooth muscle in the corpus cavernosum was detected by Masson trichrome staining.Results Cx43 is highly expressed in the corpus cavernosum of the penis,mainly on the cell membrane.Compared with the normal group,the expression ofα-SMC in AAV group and Gap27 group decreased,while the expression of Caspase-3 and Caspase-9 increased.Masson trichrome staining showed that the content of smooth muscle decreased in AAV group and Gap27 group.Conclusions Gap27′s function of the inhibition of gap junction can lead to apoptosis of smooth muscle cells.
作者
汪渊
郑丽英
王敬珅
郝志强
杨恒
李永乐
黄若晖
江波
钱彪
WANG Yuan;ZHENG Liying;WANG Jingshen;HAO Zhiqiang;YANG Heng;LI Yongle;HUANG Ruohui;JIANG Bo;QIAN Biao(Department of Urology,the First Affiliated Hospital of Gannan Medical University,Ganzhou 341000,Jiangxi,China;Department of Urology,the First Affiliated Hospital of the Medical College of Shihezi University,Shihezi 832000,Xinjiang,China)
出处
《中国性科学》
2023年第4期12-16,共5页
Chinese Journal of Human Sexuality
基金
江西省卫生健康委科技计划(SKJP420203656)
石河子大学成果转化与技术推广(CGZH201810)
赣南医学院第一附属医院博士启动基金(QD063)。
