摘要
目的探讨肉桂醛对变应性鼻炎(allergic rhinitis,AR)大鼠鼻粘膜细胞凋亡的影响及机制。方法构建大鼠AR模型,并随机分为AR组、肉桂醛低、中、高剂量组和氯雷他定组,另选10只大鼠为假手术组(sham组)。检测大鼠血清IL-6水平、鼻粘膜组织细胞凋亡和IL-6、JAK2、STAT3 mRNA表达以及JAK2、p-JAK2、STAT3、p-STAT3、Bcl-2及Bax蛋白表达。结果与sham组比,AR组大鼠血清IL-6水平、鼻粘膜组织IL-6 mRNA及p-JAK2/JAK2、p-STAT3/STAT3和Bcl-2蛋白表达均升高(P<0.05),细胞凋亡指数(AI)和Bax蛋白表达降低(P<0.05);与AR组比,肉桂醛低、中、高剂量组和氯雷他定组大鼠血清IL-6水平、鼻粘膜组织IL-6 mRNA及p-JAK2/JAK2、p-STAT3/STAT3和Bcl-2蛋白表达均降低(P<0.05),AI和Bax蛋白表达升高(P<0.05)。结论肉桂醛可促进AR大鼠鼻粘膜细胞凋亡,其机制可能与抑制IL-6/JAK2/STAT3通路有关。
Objective To investigate the effect of cinnamaldehyde on apoptosis of nasal mucosa cells in rats with allergic rhinitis(AR)and its mechanism.Methods The AR model was established in rats and the rats were randomly divided into AR group,low-,medium-and high-dose cinnamaldehyde groups and loratadine group,another 10 rats were selected as the sham operation group(sham group).The apoptosis of nasal mucosa cells,the level of serum IL-6,and the expression levels of IL-6,JAK2,STAT3 mRNAs and JAK2,p-JAK2,STAT3,p-STAT3,Bcl-2,Bax proteins were detected.Results Compared with the sham group,the level of serum IL-6,the expression levels of IL-6 mRNA and P-JAK2/JAK2,P-STAT3/STAT3 and Bcl-2 proteins of nasal mucosa cells were increased in the AR group(P<0.05),while the apoptosis index(AI)and Bax protein expression level were decreased(P<0.05).Compared with the AR group,the level of serum IL-6,the expression levels of IL-6 mRNA and P-JAK2/JAK2,P-STAT3/STAT3 and Bcl-2 proteins in rats nasal mucosa cells in the low-dose,medium-dose and high-dose cinnamaldehyde groups and the loratadine group were decreased(P<0.05),while the AI and Bax expression level were increased(P<0.05).Conclusion Cinnamaldehyde can promote the apoptosis of nasal mucosal cells in AR rats,and the mechanism may be related to the inhibition of IL-6/JAK2/STAT3 signaling pathway.
作者
谷牧
杨丽辉
GU Mu;YANG Lihui(Department of Otolaryngology,Liaoning Provincial People’s Hospital,Shenyang,110000,China)
出处
《医学分子生物学杂志》
CAS
2023年第2期160-165,共6页
Journal of Medical Molecular Biology
