摘要
该研究通过D-半乳糖建立小鼠衰老模型,观察了一株新发现的乳酸菌(Lactobacillus delbrueckii subsp.bulgaricus KSFY07,LDSBKSFY07)对衰老小鼠运动能力提升的作用并阐述了其作用机制。研究通过观察衰老小鼠跑步能力、游泳耐力、血液生化指标、组织病理学变化和mRNA表达变化、小鼠肠道内容物微生物变化来检测LDSBKSFY07提升小鼠运动能力的作用及机制。动物实验结果显示LDSBKSFY07(1.5×10^(9) CFU/kg BW灌胃)能够延长衰老小鼠的跑步时间和力竭游泳时间,降低衰老小鼠血清中的血尿素氮、血乳酸、丙二醛的水平,增加肝糖原、肌糖原的水平及超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶的酶活力。组织病理学观察提示LDSBKSFY07减轻了小鼠肝脏和肾脏肾组织的衰老损伤。qPCR结果显示LDSBKSFY07具备下调衰老小鼠肝脏、肾脏和骨骼肌组织诱导型一氧化氮合酶、肿瘤坏死因子α的mRNA表达和上调神经型一氧化氮合酶、Cu/Zn-SOD、Mn-SOD、过氧化氢酶表达的能力。另外,LDSBKSFY07还可以下调衰老小鼠骨骼肌组织的syncytin-1 mRNA表达。同时,LDSBKSFY07还增加了衰老小鼠肠道中厚壁菌门、乳酸杆菌、双歧杆菌的丰度和降低了拟杆菌门的丰度。而且,LDSBKSFY07对组织、血清和肠道菌群状态的调节作用优于维生素C(150 mg/kg BW灌胃)具有减缓小鼠衰老和提升其运动能力的效果。
In this study,the aging model of mice was established by D-galactose.The effect of a newly discovered lactic acid bacteria(Lactobacillus delbrueckii subsp.bulgaricus KSFY07,LDSBKSFY07)on the improvement of exercise ability in aging mice and its mechanism were observed.The study examined the effect and mechanism of LDSBKSFY07 on improving the exercise ability of mice by observing the running ability,swimming endurance,blood biochemical indexes,histopathological changes and mRNA expression of aging mice,and the microbial community in the intestinal of mice.The results of animal experiments showed that LDSBKSFY07(1.5×10^(9) CFU/kg BW gavage)prolonged the running time and exhaustive swimming time of aging mice,and reduced blood urea nitrogen(BUN),blood lactic acid(BLA),malondialdehyde(MDA)in the serum of aging mice,and increased the levels of hepatic glycogen(HG),muscle glycogen(MG)and the activities of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px).Histopathological observations suggested that LDSBKSFY07 alleviated the aging damage of mouse liver and kidney tissues.LDSBKSFY07 also down-regulated the expression of inducible nitric oxide synthase(iNOS),TNF-αmRNA expression and up-regulated the expression of neuronal nitric oxide synthase(nNOS),Cu/Zn-SOD,Mn-SOD,catalase(CAT)in liver,kidney,and skeletal muscle tissue of aging mice.In addition,LDSBKSFY07 down-regulated syncytin-1 mRNA expression in skeletal muscle tissue of aging mice.Meanwhile,LDSBKSFY07 increased the abundance of Firmicutes,Lactobacillus and Bifidobacterium and decreased the abundance of Bacteroides in the gut of aging mice.Moreover,LDSBKSFY07 had a better regulatory effect on the status of tissue,serum and intestinal flora than vitamin C(150 mg/kg BW by gavage),which could slow down the aging of mice and improve their exercise ability.
作者
邓潇潇
周雅琳
冯霞
赵欣
DENG Xiaoxiao;ZHOU Yalin;FENG Xia;ZHAO Xin(Collaborative Innovation Center for Child Nutrition and Health Development,Chongqing Engineering Research Center of Functional Food,Chongqing Engineering Laboratory for Research and Development of Functional Food,Chongqing University of Education,Chongqing 400067,China;College of Sports and Health Management,Chongqing University of Education,Chongqing 400067,China;Children’s Sports Health Promotion Institute,Chongqing University of Education,Chongqing 400067,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2023年第7期49-58,共10页
Food and Fermentation Industries
基金
重庆市高校创新研究群体项目(CXQTP20033)
重庆市自然科学基金面上项目(CSTB2022NSCQ-MSX1208)
重庆市教育委员会科学技术研究项目(KJQN202201609)。
