摘要
目的探讨乳腺癌患者肠道菌群丰度或者多样性的不同对乳腺癌组织中肿瘤浸润淋巴细胞(TIL)浸润程度是否有影响。方法根据肿瘤浸润淋巴细胞的浸润程度,选取2017年3~10月间在河北医科大学第四医院乳腺中心就诊、经活检病理证实为乳腺浸润性癌的女性患者80例,年龄37~74岁,分为高度浸润(TIL-H)组25例、中度浸润(TIL-M)组34例、低度浸润(TIL-L)组21例。通过Meta 16S DNA测序,对肠道菌群的DNA进行分析,比较这3组间肠道菌群丰度或者多样性的差异。肿瘤浸润淋巴细胞与临床特征之间的关系采用χ^(2)检验;肿瘤浸润淋巴细胞的浸润程度与肠道菌群的关系采用β多样性分析。结果 TIL-H组、TIL-M组和TIL-L组分别有3 174,2 996和2 877种不同的运算分类单位(OUT),3组乳腺癌患者(TIL-L、TIL-M、TIL-H)肠道菌群在加权和未加权的3组间β多样性分析中差异均具有统计学意义(加权,P<0.01;未加权,P<0.01),且在TIL-L与TIL-H组间比较时差异最大(P<0.01)。结论肿瘤浸润淋巴细胞在乳腺癌患者中的浸润水平与肠道菌群的多样性密切相关。
Objective To investigate whether the abundance or diversity of intestinal flora in breast cancer(BC)patients effects on the degree of tumor infiltration lymphocytes in breast cancer tissues.Methods Between March 2017 and October 2017,a total of 80 biopsy-confirmed female patients with BC were enrolled in the present study at the Breast Center of the Fourth Hospital of Hebei Medical University(Shijiazhuang,China),age distribution ranged from 37 to 74 years,which were divided into three groups based on the infiltration of tumor-infiltrating lymphocytes,as follows:high infiltration of TILs(TIL-H)25 cases,medium infiltration of TILs(TIL-M)34 cases and low infiltration of TILs(TIL-L)21 cases.DNA of the intestinal flora was determined by Illumina sequencing and taxonomy of 16S ribosomal RNA genes.Compare the differences in the abundance or diversity of intestinal flora between these three groups.The relationship between tumor-infiltrating lymphocytes and clinical characteristics was analyzed byχ^(2) tested,and the relationship with intestinal flora was analyzed byβdiversity.Results There were 3,174,2,996,and 2,877 different OUTs in the TIL-H,TIL-M,and TIL-L groups.Theβ-diversity distribution was statistically significant(weighted UniFrac,P<0.01;unweighted UniFrac,P<0.01)when comparing the three groups(TIL-H vs.TIL-M vs.TIL-L),the differences between TIL-L and TIL-H groups were greatest.Conclusions The infiltration level of tumor-infiltrating lymphocytes in breast cancer patients was closely related to the diversity of intestinal flora.
作者
史佳杰
张熙
程萌
张硕
耿翠芝
Shi Jiajie;Zhang Xi;Cheng Meng;Zhang Shuo;Geng Cuizhi(Breast Center,Fourth Hospital of Hebei Medical University,Shijiazhuang 050035,China)
出处
《中华医学杂志》
CAS
CSCD
北大核心
2021年第38期3164-3167,共4页
National Medical Journal of China
