摘要
目的:探讨清浊曲比亲艾拉蜜膏对牙周炎(PD)大鼠模型病理进展及血清炎症细胞因子表达的影响。方法:建立SD大鼠PD模型,将30只PD大鼠按照随机数字表法分为模型组、清浊曲比亲艾拉蜜膏治疗组和抗菌药物治疗组,每组10只,另取10只健康大鼠作为空白对照组,清浊曲比亲艾拉蜜膏治疗组大鼠予以清浊曲比亲艾拉蜜膏2.7 g/(kg·d)灌胃给药,抗菌药物治疗组大鼠予以阿莫西林胶囊135 mg/(kg·d)和甲硝唑片54 mg/(kg·d)灌胃给药,空白对照组和模型组大鼠每日予以等容积的0.9%氯化钠溶液灌胃,各组均连续给药28 d。造模前和造模给药第1、7、14、21及28日,检测各组大鼠体重、牙龈指数、牙齿松动度、牙槽骨吸收值及牙周袋深度;造模给药28 d后,采用酶联免疫吸附试验测定各组大鼠血清白细胞介素(IL)6、IL-8和肿瘤坏死因子α(TNF-α)含量,采用苏木精-伊红(HE)染色观察各组大鼠牙周组织病理学变化情况。结果:造模给药后第7、14、21及28日,与模型组比较,清浊曲比亲艾拉蜜膏治疗组和抗菌药物治疗组大鼠的体重显著升高(P<0.05),牙龈指数、牙齿松动度、牙槽骨吸收值及牙周袋深度均显著降低(P<0.05),血清IL-6、IL-8及TNF-α含量显著降低(P<0.05),差异均有统计学意义,且大鼠牙龈上皮破坏程度减小,有少量炎症细胞浸润,牙周韧带中胶原纤维排列也较为整齐。结论:使用清浊曲比亲艾拉蜜膏治疗PD大鼠,能够提高大鼠体重,降低牙龈指数、牙齿松动度、牙槽骨吸收值及牙周袋深度,并抑制血清炎症细胞因子IL-6、IL-8及TNF-α水平,减缓PD病理进程。
OBJECTIVE:To probe into the pathological progress of Qingzhuo Qubiqin Ailami paste on periodontitis(PD)and its effects on expression of serum inflammatory cytokines in a rat model.METHODS:SD rat model of PD was established,and 30 PD rats were divided into the model group,Qingzhuo Qubiqin Ailami paste treatment group and antibiotic treatment group according to the random table method,with 10 cases in each group.Another 10 healthy rats were taken as the blank control group.The Qingzhuo Qubiqin Ailami paste treatment group was given Qingzhuo Qubiqin Ailami paste 2.7 g/(kg·d)by gavage,the antibiotic treatment group received amoxicillin capsules 135 mg/(kg·d)and Metronidazole tablets 54 mg/(kg·d)by intragastric administration,and the blank control group and model group were intragastrically administered with the same volume of 0.9%sodium chloride solution normal saline every day,and each group was administered continuously for 28 d.Body mass,gingival indicator,tooth mobility,alveolar bone resorption value and periodontal pocket depth were measured before modeling and on the 1st,7th,14th,21st and 28th day after modeling.After 28 d of model administration,the levels of interleukin(IL)-6,IL-8 and tumor necrosis factor-α(TNF-α)in serum of rats in each group were determined by enzyme-linked immunosorbent assay(ELISA),hematoxylin-eosin(HE)staining was used to observe the pathological changes of periodontal tissue in each group of rats.RESULTS:On the 7th,14th,21st and 28th day after modeling,compared with the model group,the body mass of the rats in the Qingzhuo Qubiqin Ailami paste treatment group and antibiotic treatment group increased significantly(P<0.05),the gingival indicator,tooth mobility,alveolar bone resorption value and periodontal pocket depth decreased significantly(P<0.05),the levels of IL-6,IL-8 and TNF-αin serum decreased significantly(P<0.05),with statistically significant differences,the degree of damage to the gingival epithelium of the rats was reduced,a small amount of inflammatory cells were infi
作者
马涛
时婧
徐彬
MA Tao;SHI Jing;XU Bin(Dept.of Pharmacy,Urumqi Stomatological Hospital,Urumqi 830002,China;Dept.of Periodontics,Urumqi Stomatological Hospital,Urumqi 830002,China)
出处
《中国医院用药评价与分析》
2023年第3期308-311,316,共5页
Evaluation and Analysis of Drug-use in Hospitals of China
基金
新疆维吾尔自治区卫生健康青年医学科技人才专项科研项目(No.WJWY-202110)。
关键词
牙周炎
清浊曲比亲艾拉蜜膏
牙周组织
血清炎症细胞因子
Periodontitis
Qingzhuo Qubiqin Ailami paste
Periodontal tissue
Serum inflammatory cytokines
