摘要
目的观察质量分数1%阿托品对豚鼠形觉剥夺性近视(FDM)进展的防控作用及其潜在的生物学机制。方法选取屈光状态正常的3周龄三色豚鼠69只,采用随机数字表法将其随机分为正常对照组19只、FDM模型组19只、FDM+阿托品组19只和阿托品组12只。采用半透明乳胶气球遮盖右眼的方法建立FDM模型,正常对照组不进行实验干预;FDM模型组单纯遮盖右眼4周;FDM+阿托品组遮盖右眼4周,同时每日使用1%阿托品凝胶点眼1次;阿托品组每日使用1%阿托品凝胶点眼1次,共4周。分别于实验前、实验2周和实验4周时采用带状光检影镜进行屈光度测定,采用眼科A型超声仪测量眼轴长度。实验4周时采集完整眼球制作石蜡切片,光学显微镜下观察巩膜组织形态学变化;采集后极部巩膜组织,透射电子显微镜下观察巩膜组织超微结构变化;采用相对和绝对定量同位素标记(iTRAQ)联合液相色谱-串联质谱(LC-MS/MS)技术进行巩膜组织蛋白质质谱检测。结果正常对照组、FDM模型组、FDM+阿托品组和阿托品组实验眼不同时间点屈光度总体比较,差异均有统计学意义(F分组=138.892,P<0.001;F时间=167.270,P<0.001),其中FDM模型组实验2周和4周、FDM+阿托品组实验4周较正常对照组屈光度向近视化方向发展,实验2周和4周FDM+阿托品组较FDM模型组屈光度向远视化方向发展,屈光度比较差异均有统计学意义(均P<0.001)。正常对照组、FDM模型组、FDM+阿托品组和阿托品组实验眼不同时间点眼轴长度总体比较差异均有统计学意义(F分组=32.346,P<0.001;F时间=353.797,P<0.001),其中FDM模型组实验2周和4周、FDM+阿托品组实验4周眼轴长度均长于相应时间点正常对照组,FDM+阿托品组实验2周和4周眼轴长度均短于相应时间点FDM模型组,差异均有统计学意义(均P<0.01)。FDM模型组豚鼠后极部巩膜胶原纤维排列疏松且紊乱,FDM+阿托品组后极部巩膜胶原纤维排列�
Objective To observe the prevention and control effect of 1%atropine on the progression of form deprivation myopia(FDM)in guinea pigs and the potential biological mechanism.Methods Sixty-nine 3-week-old tricolor guinea pigs with normal refraction were randomly divided into a normal control group(n=19),a FDM group(n=19),a FDM+atropine group(n=19),and an atropine group(n=12).No intervention was given to guinea pigs in normal control group.The FDM model was established by covering the right eye of guinea pigs with a semitransparent latex facemask for 4 weeks in FDM and FDM+atropine groups.For the FDM+atropine group,1%atropine gel was topically administered to the form-deprived right eyes once a day for 4 weeks.For the atropine group,the right eye was treated with 1%atropine gel once a day for 4 weeks.Refraction and axial length of guinea pigs were measured by retinoscopy and ophthalmic A-scan ultrasonography respectively at baseline,experiment week 2 and week 4.In experiment week 4,eyeballs were enucleated to make sections via the paraffin wax processing procedure,and the microstructural and ultrastructural changes of the sclera were observed under the light microscope and transmission electron microscope,respectively.The isobaric tags for relative and absolute quantitation labeling combined with liquid chromatography-tandem mass spectrometry were used to identify the differentially expressed proteins.Use and care of the animals complied with the Regulation for the Administration of Affairs Concerning Experiment Animals by State Science and Technology Commission.The study protocol was approved by the Institutional Animal Care and Use Committee of Tianjin Medical University(No.TJYY2020111028).Results There were statistically significant differences in the diopter of guinea pigs at different time points among the four groups(F group=138.892,P<0.001;F time=167.270,P<0.001).Compared with normal control group,the diopter of guinea pigs in FDM group at experiment weeks 2 and 4,and FDM+atropine group at experiment week 4 d
作者
嵇霄雯
宫博腾
祝颖
鹿大千
刘琳
杜蓓
刘勋
魏瑞华
Ji Xiaowen;Gong Boteng;Zhu Ying;Lu Daqian;Liu Lin;Du Bei;Liu Xun;Wei Ruihua(Tianjin Key Laboratory of Retinal Functions and Diseases,Tianjin Branch of National Clinical Research Center for Ocular Disease,Eye Institute and School of Optometry,Tianjin Medical University Eye Hospital,Tianjin 300384,China)
出处
《中华实验眼科杂志》
CAS
CSCD
北大核心
2023年第4期303-311,共9页
Chinese Journal Of Experimental Ophthalmology
基金
天津市教委科研计划项目(2020KJ177)。
