摘要
为明确云南特色葡萄产区葡萄霜霉病菌的群体遗传结构,本研究采用7对SSR分子标记对采自云南3个地区的155株葡萄霜霉病菌进行了群体遗传多样性研究,并分析了病原菌群体遗传结构与地域之间的关系。7对引物共检测出41个等位基因,114种基因型,病原菌群体的Shannon’s信息指数和Nei’s无偏基因多样性指数分别为0.942和0.600。分子方差分析和群体连锁不平衡分析显示,不同群体间存在相当大的遗传变异。遗传分化系数、基因流以及Structure分析表明,元谋群体与宾川、寻甸两群体之间均具有较高的基因交流,宾川和寻甸两群体间则存在一定的遗传分化。上述结果表明,云南葡萄霜霉病菌群体存在较高水平的遗传多样性,且群体遗传结构与地理距离之间存在一定相关性。
To clarify the population genetic structure of Plasmopara viticola in Yunnan province,the population genetic diversity of 155 strains of Plasmopara viticola collected from three regions was studied by seven pairs of SSR markers,and the relationship between population genetic structure and region was analyzed.A total of 41 alleles and 114 distinct genotypes were identified.Shannon’s information index and Nei’s unbiased gene diversity index were 0.942 and 0.600,respectively.The analysis of molecular variance and population linkage imbalance showed that there were considerable genetic variations among different populations.Based on genetic differentiation index,gene flow and Structure analysis,Yuanmou population had a high gene flow with Binchuan and Xundian populations,while Binchuan and Xundian populations showed genetic differentiation to some extent.In conclusion,there was a high level of genetic diversity in the populations of Plasmopara viticola in Yunnan,and there was a correlation between the population genetic structure and geographical distance to some degree.
作者
王境杨
张昊
孔繁芳
周连柱
杨敏
杜飞
邓维萍
黄晓庆
朱书生
WANG Jingyang;ZHANG Hao;KONG Fanfang;ZHOU Lianzhu;YANG Min;DU Fei;DENG Weiping;HUANG Xiaoqing;ZHU Shusheng(College of Plant Protection,Yunnan Agricultural University,Key Laboratory for Agro-biodiversity and Pest Control of Ministry of Education,Kunming 650201,China;Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China)
出处
《植物保护》
CAS
CSCD
北大核心
2023年第2期170-175,226,共7页
Plant Protection
基金
国家现代农业产业技术体系建设专项(CARS-29)
国家自然科学基金(32001843)
云南农业大学林下中药材有机种植省创新团队(202105AE160016)
云南省李健强专家工作站(202105AF150046)
云南省科技计划“放管服”及稳定支持专项重点项目(202001BB050001)。
