摘要
目的:观察金水缓纤组分方Ⅱ对肺纤维化大鼠氧化/抗氧化系统的影响。方法:60只大鼠随机分为空白组、模型组、金水缓纤组分方Ⅱ组、金水缓纤组及吡啡尼酮组,每组12只。气管插管后滴注博来霉素构建肺纤维化大鼠模型。从第29天开始,除空白组及模型组灌胃羧甲基纤维素钠溶剂外,其余各组均灌胃相应药物,持续14 d。第42天结束后取肺组织进行HE、Masson染色观察病理变化,检测肺组织羟脯氨酸(HYP)、丙二醛(MDA)及SOD含量,免疫组化观察Ⅰ型胶原(Co l-Ⅰ)、Ⅲ型胶原(Co l-Ⅲ)、α-平滑肌肌动蛋白(α-SMA)、核因子E2相关因子2(Nrf2)和血红素加氧酶(HO-1)表达情况。结果:与空白组比较,模型组大鼠肺组织Szapiel和Ashcroft评分均显著增加(P<0.01),HYP、MDA含量及Col-Ⅰ、Col-Ⅲ和α-SMA表达显著升高(P<0.01),SOD含量降低(P<0.01),Nrf2、HO-1表达显著减少(P<0.05);与模型组比较,金水缓纤、金水缓纤组分方Ⅱ及吡啡尼酮均可显著降低肺组织Szapiel和Ashcroft评分,HYP含量及Col-Ⅰ、α-SMA表达,增加HO-1表达(P<0.01,P<0.05);金水缓纤方组分方Ⅱ可显著降低肺组织MDA含量及Col-Ⅲ表达、增加Nrf2表达(P<0.01,P<0.05)。结论:金水缓纤组分方Ⅱ可通过激活Nrf2抑制氧化应激改善肺纤维化。
Objective:To observe the effects of effective-component compatibility of Jinshui Huanxian Formula II on the oxidative/antioxidant system of pulmonary fibrosis in rats.Methods:A total of 60 rats were randomly divided into blank group,model group,ECC-JHF II group,Jinshui Huanxian group and pirenidone group,with 12 rats in each group.Pulmonary fibrosis rat model was established by intratracheal instillation of bleomycin.Rats in the blank group and the model group were administrated with CMC-Na by gastrogavage from 29th day after modeling,and rats in the other groups were treated with the corresponding drugs for 14 days.At the end of the 42nd day,rat lung tissues were collected for HE and Masson staining to observe the pathological changes,and the contents of HYP,MDA and the activity of SOD in lung tissues were detected;The expressions of type I collagen(Col-I),type III collagen(Col-III),α-smooth muscle actin(α-SMA),nuclear factor E2-related factor 2(Nrf2)and heme oxygenase 1(HO-1)were observed by immunohistochemistry.Results:Compared with the blank group,the scores of Szapiel and Ashcroft in model group were significantly increased(P<0.01),the content of HYP,MDA and the expressions of Col-I,Col-III andα-SMA in lung tissues were significantly increased(P<0.01),the activity of SOD and the expressions of Nrf2,HO-1 in lung tissues were significantly decreased(P<0.01,P<0.05).Compared with model group,JHF,ECC-JHF II and pirfenidone decreased the scores of Szapiel and Ashcroft,HYP content,the expression of Col-I andα-SMA,increased HO-1 expression in lung tissue(P<0.01,P<0.05);ECC-JHF II also can decreased the content of MDA and the expression of Col-I,increased the expression of Nrf2 in lung tissue(P<0.01,P<0.05).Conclusion:ECC-JHF II could ameliorate pulmonary fibrosis by activing Nrf2 to inhibit oxidative stress.
作者
殷晓红
白云苹
邵栋
赵鹏
李建生
YIN Xiao-hong;BAI Yun-ping;SHAO Dong;ZHAO Peng;LI Jian-sheng(Academy of Chinese Medicine Sciences,Henan Key Laboratory of Chinese Medicine for Respiratory Disease,Henan University of Chinese Medicine,Zhengzhou 450046,China;Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases Co-constructed by Henan Province&Education Ministry of China,Henan University of Chinese Medicine,Zhengzhou 450046,China;The First Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450000,China)
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2023年第3期1213-1217,共5页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金项目(No.81904170)
河南省重点研发与推广专项(No.212102310356)。
关键词
肺纤维化
金水缓纤组分方Ⅱ
组分配伍
氧化应激
NRF2
Pulmonary fibrosis
Effective-component compatibility of Jinshui Huanxian Formula II
Component compatibility
Oxidative stress
Nrf2
