摘要
目的比较两种不同的复合造模方法,寻找建立气阴两虚证模型的优势方法。方法72只BALB/c小鼠随机分为空白组、游泳复合组、睡眠剥夺复合组,每组24只。每组再按造模3周、6周、9周随机分为3小组,每小组8只。其中游泳复合组采用饮食不节^(+)热性药灌胃^(+)负重游泳的方法,睡眠剥夺复合组采用饮食不节^(+)热性药灌胃^(+)睡眠剥夺的方法,分别造模3周、6周、9周。造模第0周,记录每只小鼠抓力与体质量。造模第3、6、9周比较各组小鼠体质量、抓力、力竭游泳时间、饮水量、唾液流率、粪便含水率、面温;进行旷场实验,记录300 s内小鼠活动的总路程、站立次数、角落时间和中心时间;计算胸腺指数、脾脏指数;检测外周血中CD4^(+)、CD8^(+)T淋巴细胞含量和血清中环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMP)含量。结果试验期间,游泳复合组小鼠死亡3只,睡眠剥夺复合组死亡1只。造模第3周,与空白组比较,游泳复合组力竭游泳时间显著减少,睡眠剥夺复合组体重、力竭游泳时间显著减少,总路程、站立次数和中心时间显著增加(P<0.01);与游泳复合组同时间相比,睡眠剥夺复合组总路程、站立次数和中心时间均显著增加(P<0.05或P<0.01)。造模第6周,与空白组相比,游泳复合组角落时间、血清cAMP含量、cAMP/cGMP值显著增加,睡眠剥夺复合组cAMP/cGMP值显著增加,外周血CD4^(+)T淋巴细胞含量、唾液流率、胸腺指数显著降低,游泳复合组和睡眠剥夺复合组抓力、力竭游泳时间、脾脏指数显著减少(P<0.05或P<0.01)。造模第9周,与空白组相比,游泳复合组和睡眠剥夺复合组饮水量、角落时间、血清cAMP含量、cAMP/cGMP值显著增加,唾液流率、抓力、力竭游泳时间、总路程、站立次数、中心时间、血清cGMP含量、胸腺指数、脾脏指数及外周血CD4^(+)T淋巴细胞、CD8^(+)T淋巴细胞、CD4^(+)/CD8^(+)值显著减少,游泳复�
Objective To compare two different composite modeling methods and find the superior one to establish qi-yin deficiency syndrome model.Methods A total of 72 BALB/c mice were randomly divided into normal group,swimming composite model group and sleep deprivation composite model group,with 24 mice in each group.Each group was further randomly divided into 3 groups according to 3 weeks,6 weeks and 9 weeks of modeling,with 8 mice in each group.Swimming composite model group was given uncontrolled diet and drugs of heat nature by gavage plus weight-bearing swimming,while the sleep deprivation composite model group was given uncontrolled diet and drugs of heat nature by gavage plus sleep deprivation,which were modeled for 3 weeks,6 weeks and 9 weeks,respectively.At week 0 of the modelling,the grip strength and body weight of each mouse were recorded.At week 3,6,and 9,the weight,grip strength,exhaustion swimming time,water intake quantity,saliva flow rate,fecal moisture content,and surface temperature were recorded and compared between groups;open field test was used to record the total distance traveled within 300s,the standing frequency,and time spent in the center and in the corner;the thymus index and spleen index were calculated;the peripheral blood CD4^(+),CD8^(+)T lymphocytes,as well as the contents of cyclic adenosine phosphate(cAMP)and cyclic guanosine phosphate(cGMP)in serum were detected.Results During the experiment,3 mice in the swimming composite model group,and 1 mouse in the sleep deprivation composite group died.At week 3,compared to those in the normal group,the exhaustive swimming time in the swimming composite model group,as well as the weight and exhaustive swimming time in the sleep deprivation composite model group significantly decreased,while the total distance traveled,the standing frequency and the time spent in the center significantly increased in the sleep deprivation composite model group(P<0.01);the sleep deprivation composite model group had increased total distance traveled,the standing
作者
丁崧
高长久
卢芳
柳长凤
轩秀秀
刘树民
DING Song;GAO Changjiu;LU Fang;LIU Changfeng;XUAN Xiuxiu;LIU Shumin(Graduate School,Heilongjiang University of Chinese Medicine,Harbin,150040;Institute of Chinese Medicine,Heilongjiang University of Chinese Medicine)
出处
《中医杂志》
CSCD
北大核心
2023年第4期401-409,共9页
Journal of Traditional Chinese Medicine
基金
国家自然科学基金(81302898)。
关键词
气阴两虚证
证候模型
负重游泳
睡眠剥夺
qi-yin deficiency syndrome
syndrome model
weight-bearing swimming
sleep deprivation
