摘要
以藏药细果角茴香叶为材料,采用组织分离法分离纯化出一株内生真菌X-396。经形态学观察、ITS测序与序列比对鉴定内生真菌X-396为间座壳属(Diaporthe sp.)真菌Diaporthe sp.YG-2015。进一步利用气相色谱-质谱(GC-MS)联用技术对该真菌发酵物脂溶性成分进行检测与分析,并通过CCK-8法测定其抗肿瘤活性。结果表明,发酵物脂溶性成分共鉴定出35个,其中含量较高的成分为麦角甾醇(31.83%)、β-谷甾醇(12.00%)和十六酸甲酯(4.22%);体外抗肿瘤活性测试显示,发酵物脂溶性成分对人肿瘤细胞株A549、MCF-7和HepG2均具有不同程度的抑制作用,其中对HepG2抑制效果最好,IC 50为22.65μg/mL。本研究表明细果角茴香内生真菌X-396发酵物脂溶性成分中具有产生抗肿瘤活性化合物的潜力,具有进一步深入研究的价值。
An endophytic fungus X-396 was isolated from the leaves of Hypecoum leptocarpum Hook.f.et Thoms.through a tissue separating method.The endophytic fungus X-396 was identified as Diaporthe sp.YG-2015 according to the morphologic observation and ITS sequence analysis.Its fat-soluble components of fermentation were further studied with GC-MS,and antitumor activity was measured by CCK-8 assay.35 kinds of fat-soluble components were detected and identified.The findings showed that the main components of fat-soluble components in fermentation of X-396 were ergosterol(31.83%),β-stitosterol(12.00%)and methyl hexadecanoate(4.22%).Antitumor activity test in vitro showed that the fat-soluble components of fermentation had different degrees of inhibitory effects on human tumor cell lines A549,MCF-7 and HepG2,and HepG2 had the best inhibitory effect with IC 50 of 22.65μg/mL.The results indicate that the fat-soluble components of endophytic fungus X-396 fermentation have the potential to produce antitumor active compounds,and deserve to be deeply investigated.
作者
谭慧
皮立
杨小龙
安婷
郭旭栋
刘艳萍
文怀秀
TAN Hui;PI Li;YANG Xiao-long;AN Ting;GUO Xu-dong;LIU Yan-ping;WEN Huai-xiu(Qinghai Provincial Key Laboratory of Tibetan Medicine Research,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810008,China;Research Institute of Forestry,Qinghai Academy of Agriculture and Forestry,Qinghai University,Xining 810016,China;University of Chinese Academy of Sciences,Beijing 100049,China)
出处
《天然产物研究与开发》
CAS
CSCD
2023年第3期420-426,共7页
Natural Product Research and Development
基金
青海省科技厅项目(2021-SF-SW1)
“青海省藏药研究重点实验室”(2022-ZJ-Y03)。
关键词
细果角茴香
内生真菌
发酵物
脂溶性成分
GC-MS
抗肿瘤
Hypecoum leptocarpum Hook.f.et Thoms.
endophytic fungus
fermentation
fat-soluble components
GC-MS
antitumor
