摘要
从霍山石斛(Dendrobium huoshanense)根、茎、叶中筛选具有抑菌活性的真菌,并对活性菌株进行种属鉴定。采用组织块法分离野生霍山石斛组织内的内生真菌;采用琼脂块法与牛津杯法研究了霍山石斛内生真菌的抑菌活性;通过形态特征及分子生物学方法鉴定菌种。结果表明,从霍山石斛组织内共分离得到的52株内生真菌中,根部数量最多,茎部次之,叶中最少;有5株内生真菌对指示菌株表现出拮抗活性,占总分离菌株的9.6%,其中菌株DH10对4种指示菌有抑菌活性,且对金黄色葡萄球菌的抑菌能力最强,其抑菌圈直径为23.73mm。根据菌株DH10的形态学特征与ITS序列系统发育分析,最相似菌株为丝枝蜡蚧霉菌(Lecanicillium aphanocladii)。综上所述,野生霍山石斛组织内内生真菌较丰富,在宿主中的数量分布存在一定的组织差异性,高活性菌株为蜡蚧菌属,可供进一步深入研究。
An antimicrobial endophytic fungus strain was isolated from the root of D. huoshanense,and its phylogenetic position was also investigated. Endophytic fungi were isolated from the healthy roots,stems and leaves of D. huoshanense by tissue inoculation culture. Antimicrobial activities of endophytic fungi were determined by the agar block method and oxford cup method,and then an active strain was identified by morphological characters and molecular methods. Results showed that a total of 52 strains of endophytic fungi were isolated from the host,and the most isolates came from the roots,then from stems,and leaves as the least; 5 of them had antimicrobial activity to the test strains which accounts for9. 6% of all strains. Among the 5 strains,the strain DH10 was found to produce antibiotic substances which showed significant inhibitory effects on the test strains,especially Staphylococcus aureus,with a formed inhibition zone of 23. 73 mm. Based on the morphological characters and ITS sequence analysis,the strain DH10 was most similar with L. aphanocladii. In summary,our research showed the abundance of endophytes from the wild D. huoshanense,and the distribution of endophytes also had a certain degree of tissue preference. The strong active strain of Lecanillium can be further studied.
作者
闵长莉
闵运江
徐慧欣
汪学军
MIN Chang-li;MIN Yun-jiang;XU Hui-xin;WANG Xue-jun(College of Biotechnology and Pharmaceutical Engineering,West Anhui University;Research Center for Endophytic Fungi Resources of Dabie Mountain,West Anhui University,Lu’an 237012,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2018年第6期968-973,共6页
Natural Product Research and Development
基金
安徽省自然科学基金(1708085MH226)
安徽省高校自然科学基金重大项目(KJ2014ZD33)
安徽省高等学校省级自然科学研究重点项目(KJ2015A172)
国家级大学生创新创业项目(201710376010)
关键词
霍山石斛
内生真菌
抑菌活性
分离
丝枝蜡蚧霉菌
Dendrobium huoshanense
endophytic fungi
antimicrobial activity
isolation
Lecanicillium aphanocladii
