摘要
以溶胶-凝胶法合成双模型介孔SiO_(2)材料,并以该材料为载体吸附固定化脂肪酶。通过BJH法孔径分析和XRD表征表明,制备的载体呈现出孔径约为3nm和30nm的硅基结构的特征。以考马斯亮蓝法考察固载率,当固定化时间为2h、缓冲溶液pH值为8、固载温度为40℃时,固定化脂肪酶的固载率最大,可达到72.9%。
The bimodel mesoporous silica was synthesized by sol-gel method,and lipase was adsorbed and immobilized on this material.The analysis by BJH method and XRD characterization showed that the prepared support exhibited the characteristics of silicon-based structure with pore size of about 3nm and 30nm.Bradford method was used to investigate the immobilization rate.When the immobilization time was 2h,the pH of the buffer solution was8,and the immobilization temperature was 40℃,the immobilization rate was the highest,reaching 72.9%.
作者
高丰琴
曹妙泷
韩玮炜
马娟
刘洋
GAO Feng-qin;CAO Miao-shuang;HAN Wei-wei;MA Juan;LIU Yang(College of Chemistry and Chemical Engineering,Xianyang Normal University,Xianyang 712000,China)
出处
《化学工程师》
CAS
2023年第3期5-7,共3页
Chemical Engineer
基金
陕西省教育厅科研计划项目(22JK0605
16JK1827)
咸阳师范学院教学改革研究项目(2019Y026
2021Y015)
咸阳师范学院(国家级)大学生创新创业训练计划项目(202210722022)。
