摘要
目的观察电针对硫酸卡那霉素慢性损害后豚鼠听皮层葡萄糖代谢的影响并探讨相关机制。方法105只成年豚鼠随机分为对照组、模型组和电针组。对照组每日颈背部皮下注射生理盐水(500 mg·kg^(﹣1)),连续7 d;模型组和电针组注射硫酸卡那霉素(500 mg·kg^(﹣1)),连续7 d,电针组在每次注射结束30 min后予电针翳风及听宫穴;两组根据观察的时间不同,分为第1、第7、第14、第28、第56、第70和第140天7组。应用正电子发射断层显像(positron emission tomography,PET)/X线计算机体层成像(computer tomography,CT)技术观察豚鼠听皮层葡萄糖代谢功能的变化规律,酶化学法检测乳酸脱氢酶(lactate dehydrogenase,LDH)和琥珀酸脱氢酶(succinate dehydrogenase,SDH)活性。结果与对照组比较,模型组第140天组豚鼠听皮层与小脑标准摄入值(standard uptake value,SUV)最大值的比值的平均比值增大(P<0.05);与模型组相比较,电针组第28和第56天组豚鼠听皮层与小脑SUV最大值的比值的平均比值增大(P<0.05)。与对照组比较,模型组第1、第14和第28天组豚鼠听皮层LDH活性升高(P<0.05),第7、第56、第70、第140天组豚鼠听皮层LDH活性无明显变化(P>0.05);与模型组比较,电针组第1、第14和第28天组豚鼠听皮层LDH活性降低(P<0.05)。与对照组比较,模型组豚鼠听皮层SDH活性变化差异无统计学意义(P>0.05);与模型组比较,电针组第7、第14、第28、第56天组豚鼠听皮层SDH活性升高(P<0.05)。结论硫酸卡那霉素慢性损害后,豚鼠听皮层葡萄糖代谢功能可发生重塑;电针可能通过减弱LDH活性及增强SDH活性,减少葡萄糖的无氧酵解,增加有氧代谢,提高葡萄糖代谢水平,促进硫酸卡那霉素慢性损害后豚鼠听皮层葡萄糖代谢功能的重塑进程。
Objective To observe the effect of electroacupuncture on glucose metabolism in the auditory cortex of guinea pigs with chronic damage caused by Kanamycin sulfate and discuss the relevant mechanism.Method A total of 105 adult guinea pigs were randomly allocated to a control group,a model group,and an electroacupuncture group.The control group received daily subcutaneous injections of normal saline on the neck and back for 7 d(500 mg·kg^(﹣1)).The model and electroacupuncture groups received an injection of Kanamycin sulfate for 7 consecutive days(500 mg·kg^(﹣1)).Guinea pigs in the electroacupuncture group received electroacupuncture at Yifeng(TE17)and Tinggong(SI19)once a day 30 min after each injection.According to the observation time,the model and electroacupuncture groups were further divided into 7 groups,including 1 d,7 d,14 d,28 d,56 d,70 d,and 140 d groups.The positron emission tomography/computer tomography(PET/CT)technique was used to observe the changes in glucose metabolism in the auditory cortex of guinea pigs,and enzyme chemistry was used to measure the activities of lactate dehydrogenase(LDH)and succinate dehydrogenase(SDH).Result Compared with the control group,the average ratio of the maximum standard uptake value(SUV)of the auditory cortex to the cerebellum of guinea pigs increased in the 140 d model group(P<0.05);compared with the model group,the average ratio of the maximum SUV of the auditory cortex to the cerebellum increased in the 28 d and 56 d electroacupuncture groups(P<0.05).Compared with the control group,the LDH activity in the auditory cortex of guinea pigs increased in the 1 d,14 d,and 28 d model groups(P<0.05),while the activity in the 7 d,56 d,70 d,140 d model groups did not show significant changes(P>0.05).Compared with the model group,the LDH activity in the guinea pig’s auditory cortex decreased in the 1 d,14 d,and 28 d electroacupuncture groups(P<0.05).Compared with the control group,guinea pigs in the model group did not show significant differences in the SDH activity
作者
杨丽
陈茜
陈灵均
杨烨
殷泽登
YANG Li;CHEN Qian;CHEN Lingjun;YANG Ye;YIN Zedeng(The Affiliated Hospital of Southwest Medical University,Luzhou 646000,China;Sichuan Mianyang 404 Hospital,Mianyang 621000,China;Southwest Medical University,Luzhou 646000,China)
出处
《上海针灸杂志》
CSCD
2023年第3期300-305,共6页
Shanghai Journal of Acupuncture and Moxibustion
基金
泸州市科技局项目(2012-S-37)
宜宾市卫生局项目(201426)。
关键词
听皮层
糖代谢
耳毒性
硫酸卡那霉素
电针
乳酸脱氢酶
琥珀酸脱氢酶
Auditory cortex
Carbohydrate metabolism
Ototoxicity
Kanamycin sulfate
Electroacupuncture
Lactate dehydrogenase
Succinate dehydrogenase
