摘要
目的 分析口腔鳞状细胞癌(OSCC)患者唾液miR-15b和组织cleaved caspase-3表达及与预后的关系。方法 收集2016年5月至2020年6月期间我院收治的OSCC患者154例(OSCC组),另收集103例健康志愿者作为对照组。分别采用实时荧光定量PCR(qPCR)及免疫组化染色法检测唾液miR-15b水平及组织cleaved caspase-3表达。受试者工作特征(ROC)曲线分析唾液miR-15b表达诊断OSCC的效能。分析唾液miR-15b表达与口腔鳞癌临床病理特征的关系,采用多因素Cox回归分析影响OSCC预后的因素。结果 OSCC患者唾液miR-15b水平显著低于对照组(0.72±0.33 vs. 1.00±0.23,P<0.001)。ROC曲线分析唾液miR-15b水平诊断OSCC的曲线下面积为0.760(0.703~0.817)。与癌旁组织比较,OSCC组织中caspase-3、cleaved caspase-3蛋白表达水平显著升高(P<0.001)。经Spearman相关性分析,OSCC患者的唾液miR-15b水平与OSCC组织cleaved caspase-3蛋白表达呈负相关(r=-0.321,P<0.001)。唾液miR-15b表达与肿瘤直径和预后有关(P<0.05)。miR-15b低表达组患者5年DFS率和5年OS率分别为28.57%和44.16%,高表达组患者5年DFS率和5年OS率分别为62.34%和81.82%,差异有统计学意义(P<0.001)。多因素Cox风险比例回归模型结果显示TNM分期、根治性手术、唾液miR-15b水平均是影响OSCC患者OS的独立预后因素(P<0.05)。结论 OSCC患者唾液miR-15b水平与组织cleaved caspase-3相关,可作为预测OSCC患者预后的生物标志物。
Objective To analyze the relationship between saliva miR-15b and cleaved caspase-3 expression and prognosis in oral squamous cell carcinoma(OSCC). Methods One hundred and fifty-four patients with OSCC admitted to our hospital from May 2016 to June 2020(OSCC group) were collected, and 103 healthy volunteers were collected as control group. The levels of miR-15b in saliva and cleaved caspase-3 expression in tissues were detected by quantitative real-time PCR(qPCR) and immunohistochemical staining, respectively. The receiver operating characteristic(ROC) curve was used to analyze the diagnostic efficacy of saliva miR-15b expression in OSCC. The relationship between miR-15b expression in saliva and clinicopathologic features of oral squamous cell carcinoma was analyzed. Multivariate Cox regression was used to analyze the prognostic factors of OSCC. Results The level of miR-15b in saliva of OSCC patients was significantly lower than that of control group(0.72±0.33 vs. 1.00±0.23, P<0.001). The area under the ROC curve of saliva miR-15b to diagnose OSCC was 0.760(0.703-0.817). Expression levels of caspase-3 and cleaved caspase-3 protein were significantly higher in OSCC than in paracancer tissue(P<0.001). Spearman correlation analysis showed that saliva miR-15b levels in OSCC patients were negatively correlated with cleaved caspase-3 protein expression(r=-0.321, P<0.001). Salivary miR-15b expression was associated with tumor diameter and prognosis(P<0.05). The 5-year DFS rate and 5-year OS rate in the low expression group were 28.57% and 44.16%, respectively, while the 5-year DFS rate and 5-year OS rate in the high expression group were 62.34% and 81.82%, respectively, and the difference was statistically significant(P<0.001). Multivariate Cox proportional regression model showed that TNM stage, radical surgery, and saliva miR-15b level were independent prognostic factors affecting OS in OSCC patients(P<0.05). Conclusion The level of miR-15b in saliva of OSCC patients is correlated with cleaved caspase-3, which can be
作者
谷爱玲
何巍
安志强
GU Ailing;HE Wei;AN Zhiqing(Department of Stomatology,Puyang People s Hospital,Puyang 457000,China)
出处
《临床肿瘤学杂志》
CAS
2023年第2期127-133,共7页
Chinese Clinical Oncology
