摘要
目的:探讨基质金属蛋白酶-9(MMP-9)、环氧化物酶-2(COX-2)、细胞核因子KB(NF-κB)在口腔鳞状细胞癌(OSCC)组织中的表达及意义。方法:分别采用实时荧光定量聚合酶链反应(qRT-PCR)、蛋白免疫印迹法(Western blot)、免疫组化法检测80例OSCC组织中的表达水平;采用Pearson法分析OSCC组织中MMP-9、COX-2、NF-κB表达的相关性;OSCC患者进行3年随访,采用Kaplan-Meier对生存情况进行分析,Log-Rank法检测组间生存差异;COX回归分析影响患者预后的危险因素。结果:与正常组比较,OSCC组织中MMP-9、COX-2、NF-κB mRNA及蛋白水平显著升高(P<0.05);与正常组相比,OSCC组MMP-9、COX-2、NF-κB蛋白阳性表达增强(P<0.05);MMP-9与COX-2呈正相关(r=0.356,P=0.012),MMP-9与NF-κB呈正相关(r=0.312,P=0.005),COX-2与NF-κB呈正相关(r=0.619,P=0.000);MMP-9、COX-2、NF-κB表达与OSCC患者病理分级、分化程度、淋巴结转移及TNM分期密切相关(P<0.05);MMP-9阴性表达组总体生存率高于阳性表达组(P<0.05),COX-2阴性表达组总体生存率高于阳性表达组(P<0.05),NF-κB阴性表达组总体生存率高于阳性表达组(P<0.05);COX回归分析显示淋巴结转移、TNM分期、MMP-9、COX-2、NF-κB可能是影响OSCC患者预后的独立危险因素(P<0.05)。结论:MMP-9、COX-2、NF-κB在口腔鳞状细胞癌组织中呈高表达,其表达量与患者临床病理参数密切相关。
Objective:To explore the expression and significance of MMP-9,COX-2,and NF-κB in oral squamous cell carcinoma(OSCC)tissues.Methods:qRT-PCR,western blot,and immunohistochemistry were used to detect the expression levels in 80 cases of OSCC tissues.Correlation of MMP-9,COX-2,and NF-κB in OSCC tissues was analyzed using the Pearson method.OSCC patients were followed for 3 years,Kaplan-Meier was used to analyze the survival,and Log-Rank method was used to detect the survival differences between groups.COX regression analysis was used to detect the risk factors affecting the prognosis of patients.Results:Compared with the normal group,the levels of MMP-9,COX-2,NF-κB mRNA and protein in OSCC tissues were significantly increased(P<0.05).Compared with the normal group,the positive expressions of MMP-9,COX-2,and NF-κB proteins in the OSCC group were enhanced(P<0.05).MMP-9 was positively correlated with COX-2(r=0.356,P=0.012)and NF-κB(r=0.312,P=0.005),and COX-2 was positively correlated with NF-κB(r=0.619,P=0.000).MMP-9,COX-2,and NF-κB were closely related to the pathological grade,degree of differentiation,lymph node metastasis,and TNM stage in OSCC patients(P<0.05).The overall survival rate of the MMP-9 negative expression group was higher than that of the positive expression group(P<0.05).The overall survival rate of the COX-2 negative expression group was higher than that of the positive expression group(P<0.05),and the overall survival rate of the NF-κB negative expression group was higher in the positive expression group(P<0.05).COX regression analysis showed that lymph node metastasis,TNM staging,MMP-9,COX-2,and NF-κB may be independent risk factors affecting the prognosis of OSCC patients(P<0.05).Conclusion:MMP-9,COX-2,and NF-κB were highly expressed in OSCC tissues.The expression levels of MMP-9,COX-2,and NF-κB were closely related to the clinicopathological parameters of patients.
作者
张旭
刘佳文
ZHANG Xu;LIU Jiawen(Wuhan Wuchang Hospital, Wuchang Hospital Affiliated to Wuhan University of Science and Technology, Wuhan 430000, China;Hubei University of Chinese Medicine, Wuhan 430061, China)
出处
《口腔医学研究》
CAS
CSCD
北大核心
2021年第4期360-365,共6页
Journal of Oral Science Research
