摘要
目的:探讨人参皂苷Rb1(Gs-Rb1)对慢性心力衰竭(CHF)大鼠心脏能量代谢的影响,以及腺苷酸活化蛋白激酶(AMPK)在其中的作用。方法:将阿霉素(Adr)诱导的心力衰竭大鼠随机分为CHF组、Gs-Rb1组、腺嘌呤9-β-D-阿拉伯呋喃糖苷(AraA)-1组、AraA-2组(AraA+Gs-Rb1),5’-氨基咪唑-4-甲酰胺核苷(Aicar)-1组和Aicar-2组(Aicar+Gs-Rb1),每组5只,另外选取5只健康大鼠作为对照组。除CHF组和对照组腹腔注射等体积生理盐水外,余五组分别予相应药物腹腔注射。干预结束并经心脏超声心动图检查左心室射血分数(LVEF)后,分离左心室心肌组织并分别测定心肌细胞AMPK活性和高能磷酸盐底物水平。结果:与CHF组比较,Gs-Rb1组、Aicar-1组和Aicar-2组LVEF均显著升高(P<0.05),但AraA-1组、AraA-2组显著降低(P<0.05)。与CHF组比较,AraA-1组、AraA-2组AMPK活性显著降低(P<0.05),Gs-Rb1组、Aicar-1组和Aicar-2组AMPK活性均显著升高(P<0.05)。与CHF组比较,Gs-Rb1组、Aicar-1组和Aicar-2组的磷酸肌酸(PCr)、二磷酸腺苷(ADP)和三磷酸腺苷(ATP)水平均显著升高(P<0.05),且ATP/PCr显著提高(P<0.05),而ADP/ATP均显著降低(P<0.05)。结论:Gs-Rb1可能通过AMPK途径改善衰竭心脏的能量代谢,进而改善衰竭心脏的功能。
Objective:To investigate the effect of ginsenoside Rb1(Gs-Rb1) on cardiac energy metabolism in rats with chronic heart failure(CHF),and the role of adenylate activated protein kinase(AMPK) in it. Methods:The heart failure rats which were injected Adriamycin(Adr) were randomly grouped into CHF group,Gs-Rb1 group,Adenine 9-β-DArafuran glycoside(AraA)-1 group,AraA-2 group(AraA+Gs-Rb1),5’-aminoimidazol-4-formamide nucleoside(Aicar)-1 group and Aicar-2 group(Aicar+Gs-Rb1),with 5 rats in each group. Age-matched 5 healthy rats were served as control group. Except for the CHF group and the control group,which were injected intraperitoneally with the same volume of normal saline,the other five groups were injected intraperitoneally with corresponding drugs. After the intervention and left ventricular ejection fraction(LVEF) checked by echocardiography,the left ventricular myocardial tissues were separated and AMPK activity and high energy phosphate substrate levels of myocardial cells were measured. Results:Compared with CHF group,the LVEF of Gs-Rb1,Aicar-1 and Aicar-2 groups was significantly increased(P< 0.05),but that of AraA-1 and AraA-2groups was significantly decreased(P< 0.05). Compared with CHF group,the activity of AMPK in AraA-1and AraA-2 groups was significantly decreased(P< 0.05),and the activity of AMPK in Gs-Rb1,Aicar-1 and Aicar-2 groups was significantly increased(P< 0.05). Compared with CHF group,the levels of phosphocreatine(PCr),adenosine diphosphate(ADP) and adenosine triphosphate(ATP) in Gs-Rb1,Aicar-1 and Aicar-2groups were significantly increased(P< 0.05),and the ATP/PCr was significantly increased(P< 0.05),and the ADP/ATP was significantly decreased(P< 0.05). Conclusion:Gs-Rb1 may improve the cardiac energy metabolism and function of the failing heart,which may be mediated by AMPK pathway.
作者
张娜
朱芳
孔宏亮
赵雨婷
ZHANG Na;ZHU Fang;KONG Hongliang;ZHAO Yuting(People’s Hospital of Liaoning Province,Shenyang 110016,China;Affiliated Xinhua Hospital of Dalian University,Dalian 116021,China)
出处
《山东中医药大学学报》
2023年第2期202-206,共5页
Journal of Shandong University of Traditional Chinese Medicine
基金
辽宁省科学技术基金项目(编号:20180550245)
沈阳市科技创新资金应用基础研究专项(编号:19-112-4-057)。
关键词
人参皂苷RB1
慢性心力衰竭
腺苷酸活化蛋白激酶
心肌高能磷酸盐底物
心脏能量代谢
三磷酸腺苷
大鼠
ginsenoside Rb1
chronic heart failure
adenylate activated protein kinase
myocardial high energy phosphate substrate
cardial energy metabolism
adenosine triphosphate
rat
