摘要
研究两种从盘花垂头菊中分离出的高含氧甜没药烯型倍半萜(HOBS)化合物对肿瘤细胞的影响.结果显示HOBS能够诱导人肝癌细胞SMMC-7721凋亡.单细胞电泳实验证明HOBS能够以剂量依赖的方式诱导SMMC-7721细胞损伤,流式细胞技术显示HOBS能够以剂量依赖的方式诱导SMMC-7721细胞凋亡,凋亡率达到38.9%,c_i(Ca^(2+))的测定显示HOBS能够诱导c_i(Ca^(2+))升高,所有的结果证明HOBS能够诱导人肝癌细胞SMMC-7721凋亡.提示这两种化合物有抗人肝癌细胞的SMMC-7721活性,为抗肿瘤药物的设计提供了新的思路.
The effects of two novel highly oxygenated bisabolane-type sesquiterpenes （HOBS） isolated from C. Discoideura （Cremanthodiurn discoidiem） on tumor cells were investigated. The results indicate that HOBS induced DNA damage and apoptosis of SMMC-7721 cells both in a dose-dependent manner as revealed by single cell gel electrophoresis and flow cytometry analyses, and the apoptosis rate was up to 38.9%. Measurement of ci（Ca^2＋） showed that HOBS could induce elevation of Ca^2＋. All the results suggest that HOBS could induce SMMC-7721 cells apoptosis.
出处
《兰州大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第6期49-54,共6页
Journal of Lanzhou University(Natural Sciences)
基金
Supported by the Science and Technology Support Project of Gansu Province (0708NKCA124).
