摘要
目的比较来自陕西省内的三个不同产地山茱萸果核的抗氧化活性,为山茱萸果核资源的综合开发提供参考。方法以汉中市宁强县、汉中市洋县和宝鸡市凤县三个产地的山茱萸果核为原料,分别以石油醚、无水乙醇和水为提取溶剂,通过测定上述不同提取部位对1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基的清除能力评价其抗氧化活性。结果来自宁强县、凤县和洋县的山茱萸果核石油醚部位清除DPPH自由基能力较低;无水乙醇部位清除DPPH自由基的IC50值分别为0.0038 mg·mL^(-1),0.0231 mg·mL^(-1),0.0207 mg·mL^(-1);水部位清除DPPH自由基的IC50值分别0.0134 mg·mL^(-1),0.0437 mg·mL^(-1),0.0250 mg·mL^(-1)。结论来自三个产地的山茱萸果核的三个提取部位均有抗氧化活性,其中汉中市宁强县山茱萸果核的无水乙醇提取部位活性最佳。
Objective To compare the antioxidant activity of cornus officinalis from three different areas in Shaanxi Province,and provide reference for the comprehensive development of cornus officinalis seed resources.Methods Fructus corni Fruit Core from Ningqiang County,Yangxian County,Hanzhong City and Fengxian County,Baoji City were used as raw materials,and petroleum ether,anhydrous ethanol and water were used as extraction solvents respectively.The antioxidant activity of the extracts was evaluated by measuring the scavenging ability of the different extracts to 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)free radicals.Results The petroleum ether fraction of Fructus corni Fruit Core from Ningqiang County,Fengxian County and Yangxian County had low ability to scavenge DPPH free radicals;The IC50 values of DPPH free radical scavenging in anhydrous ethanol were 0.0038 mg·mL^(-1),0.0231 mg·mL^(-1) and 0.0207 mg·mL^(-1),respectively;The IC50 values of DPPH free radical scavenging in water were 0.0134 mg·mL^(-1),0.0437 mg·mL^(-1) and 0.0250 mg·mL^(-1),respectively.Conclusion The three extracts of Fructus corni Fruit Core from three producing areas have antioxidant activity,and the ethanol extract of cornus officinalis from Ningqiang County,Hanzhong City has the best activity.
作者
张婷
姜海慧
冯石卜
宋小妹
姜祎
张化为
邓翀
ZHANG Ting;JIANG Haihui;FENG Shibu;SONG Xiaomei;JIANG Yi;ZHANG Huawei;DENG Chong(Shaanxi University of Chinese Medicine,Shaanxi Xianyang 712046,China;Shaanxi Provincial Key Laboratory of Basic and New Drug Research of Chinese Medicine,Shaanxi Xianyang 712046,China)
出处
《陕西中医药大学学报》
2023年第2期23-26,共4页
Journal of Shaanxi University of Chinese Medicine
基金
陕西省科技厅重点科研计划项目(2021SF-367)
陕西省教育厅重点科研计划项目(18JS028)
陕西中医药大学学科创新团队项目(2019-YL12)。
关键词
山茱萸
果核
抗氧化活性
提取部位
Cornus officinalis Sieb.et Zucc.
Core
Antioxidant activity
Extraction site
