摘要
为研究WWOX在人弥漫性大B淋巴瘤(DLBCL)中的作用,首先对TCGA数据库中人WWOX(hWWOX)在DLBCL样本中的表达进行分析;随后在B细胞淋巴瘤细胞株Romas、OCI-Ly7(Ly7)、38B9及临床组织样本中对上述生物信息学分析结果进行验证;通过构建稳定过表达hWWOX的淋巴瘤细胞系进一步分析hWWOX过表达对DLBCL细胞增殖的影响;最后对该细胞内Jak2-Stat3通路的活化水平进行探究。结果表明:TCGA数据库DLBCL中hWWOX高表达并在B细胞淋巴瘤细胞株中得到验证;IHC结果显示,53%的DLBCL患者组织中hWWOX高表达。Western blot结果显示,过表达hWWOX的细胞系Ly7-hWWOX得到成功构建,MTT试验表明该稳转细胞株的增殖能力较对照组显著增强,且细胞中p-Jak2、p-Stat3水平显著上调。综上,hWWOX在DLBCL中主要通过上调Jak2-Stat3信号通路而促进癌细胞增殖,这为DLBCL的临床研究和治疗提供靶标。
In order to analyze the role of WWOX in diffuse large B-cell lymphoma(DLBCL),the data obtained from TCGA database were analyzed for the expression of hWWOX in DLBCL.Western blot was used to analyze WWOX protein level expressed in B-cell lymphoma cells lines:Romas,OCI-Ly7(Ly7)and 38B9 compared with normal human lymphocytes;Immunohistochemistry(IHC)assay was utilized to analyze hWWOX expressed in DLBCL tissues and its adjacent tissues.The recombinant lentivirus containing hWWOX were packaged to infect DLBCL cell line Ly7 for the construction of cell line Ly7-hWWOX which could stably over-express hWWOX.Subsequently,the effect of WWOX on cell proliferation of Ly7-hWWOX was analyzed by MTT assay.The activation levels of intracellular signaling molecules p-Jak2,p-Stat3,Jak2,and Stat3 were analyzed.The results based on TCGA database showed that hWWOX was highly expressed in DLBCL tissues,the same results were got in B cell lymphoma cell lines:38B9 and Romas analyzed by Western blot;Results of IHC analysis showed that hWWOX was highly expressed in 53%DLBCL tissues compared with its adjacent tissues.The stable recombinant DLBCL cell line Ly7-hWWOX overexpressing hWWOX was successfully constructed and its proliferative ability was significantly enhanced by MMT assay;The result of Western blot showed that phosphorylated Jak2 and Stat3 were up-regulated significantly.It indicates that hWWOX may work as a tumor promoter in DLBCL by up-regulating Jak2-Stat3 signaling pathway and would be a useful target for DLBCL treatment and research work.
作者
许康节
詹万达
万晓洁
王杰
戴华
XU Kangjie;ZHAN Wanda;WAN Xiaojie;WANG Jie;DAI Hua(College of Medicine(Institute of Translational Medicine),Yangzhou University,Yangzhou 225009,China;Jiangsu Key Laboratory of Zoonosis,Yangzhou 225009,China;Deparment of Pathology,Affiliated Hospital of Yangzhou University,Yangzhou 225009,China;Jiangsu Key Laboratory of Experimental&Translational Non-coding RNA Research,Yangzhou 225009,China)
出处
《扬州大学学报（农业与生命科学版）》
CAS
北大核心
2022年第6期80-86,共7页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
江苏省高校自然科学研究重大项目(20KJA320005)
江苏省人兽共患病学重点实验室开放课题(R2015)。
