摘要
在籼稻和粳稻中分别选取耐盐和盐敏感的2个品种,通过对50 mmol·L^(-1)NaCl盐胁迫7 d后的幼苗植株生物量、生理参数及根尖Na^(+)、K^(+)流速的测定,研究耐盐能力不同的水稻幼苗对短期盐胁迫响应的差异。结果表明:盐胁迫下供试水稻品种幼苗植株生长均受到抑制,但与盐敏感品种相比,盐胁迫后耐盐品种植株鲜重降幅较小,体内可溶性糖和脯氨酸含量升高,而丙二醛含量降低,生长状况总体较好。利用非损伤微测技术对根尖Na^(+)、K^(+)离子流的测定结果表明,Na^(+)和K^(+)吸收和积累与耐盐性有关,且存在品种间差异。盐胁迫后,籼稻中广盐1号表现为吸收Na^(+)、K^(+)并积累在地上部,以调节地上部Na^(+)/K^(+)比;而粳稻中盐稻12号K^(+)损失量较大,但根部能减少Na^(+)吸收量,以维持根部较低的Na^(+)/K^(+)比。综上,不同类型水稻抵御盐胁迫的策略存在差异,这为利用生理指标与非损伤技术结合鉴定水稻耐盐能力提供了依据。
In order to study the physiological responses of rice seedlings with different salt tolerance to short-term salt stress,two salt-tolerant and salt-sensitive varieties,representing indica and japonica rice respectively,were selected and solution-cultured.The biomass,physiological parameters,Na^(+),K^(+)content of rice seedlings and ion flux in root tip after 7 days of 50 mmol·L^(-1)NaCl stress were measured.The results showed that the fresh weight of salt-tolerant varieties decreased less under salt stress,compared with that of salt-sensitive varieties.After salt stress,the contents of soluble sugar and proline in seedlings of salt-tolerant varieties were higher than those of salt-sensitive varieties,but malondialdehyde content was lower.The overall growth status of salt tolerant rice seedling was less affected by salt stress.Na^(+)and K^(+)flux in root tips monitored by none-invasive micro-test tTechnology(NMT)indicated that Na^(+)and K^(+)uptake by root and accumulation in rice seedlings were associated with salt tolerance and related to variety types.Under salt stress,Na^(+)and K^(+)were absorbed and accumulated in shoot of indica Guangyan 1 to regulate shoot Na^(+)/K^(+)ratio,while japonica Yandao 12 lose K^(+)but the Na^(+)uptake in root was reduced to maintain the lower Na^(+)/K^(+)ratio in root.These results suggested that the strategy in resistance to salt stress may genetically differ with rice varieties.This study provided a reference for the rapid identification and screening of salt tolerant rice materials by the integration of physiological index and root tip ion flux measurement by NMT technique.
作者
赵怡琳
吕铭
周宇熙
王燚敏
徐璐
申超
裔嗣强
柏彦超
单玉华
ZHAO Yilin;LüMin;ZHOU Yuxi;WANG Yimin;XU Lu;SHEN Chao;YI Siqiang;BAI Yanchao;SHAN Yuhua(College of Environmental Science and Engineering,Yangzhou University,Yangzhou 225127,China)
出处
《扬州大学学报（农业与生命科学版）》
CAS
北大核心
2022年第6期13-20,共8页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
国家自然科学基金面上项目(31872179)
江苏省(扬州大学)研究生科研与实践创新计划项目(KYCX21-3253)。
关键词
籼稻
粳稻
耐盐性
非损伤微测技术
indicarice
japonicarice
salt resistance
none-invasive micro-test technology
