摘要
长链非编码RNA在植物抵御逆境胁迫中起着重要作用.适宜的内参基因是准确评价其表达水平的基础.本研究利用实时荧光定量PCR技术检测16个青花菜LncRNAs表达水平,并通过geNorm、Norm-Finder、BestKeerper软件进行表达稳定性分析.结果表明:青花菜的16个LncRNAs在不同逆境胁迫下表达稳定性存在差异.其中在弱光胁迫条件下,XLOC_000400表达最稳定;在低温胁迫条件下XLOC_030832基因稳定性最好;在干旱和渍水胁迫条件下最稳定表达的内参基因分别是XLOC_007087和XLOC_012179;高温和盐胁迫处理下最稳定表达的内参基因均为XLOC_007980.XLOC_010342和XLOC_007980在青花菜不同逆境胁迫下的表达稳定性较好.研究结果可为准确定量青花菜不同逆境胁迫下的LncRNAs提供合适的内参基因.
Longnon-coding RNAs(LncRNAs)plays important role in plant resistance to stress.Appropriate reference genes are the basis to accurately evaluate their expression levels.In this study,real-time fluorescent quantitative PCR technology was used to detect the expression levels of 16 LncRNAs in broccoli,and their expression stability was analyzed by geNorm,NormFinder,and BestKeerper software.The results showed that 16 LncRNAs revealed diverse expression stability under different stresses in broccoli.Among them,XLOC_000400 was the most stable under low light stress;XLOC_030832 had the best stability under low temperature stress;the most stable reference genes under drought and waterlogging stress were XLOC_007087 and XLOC_012179,respectively;under high temperature and salt stress,the most stably reference gene was XLOC_007980.In conclusion,XLOC_010342 and XLOC_007980 have good expression stability under different stresses in broccoli.This study provides suitable reference genes for further research on the expression of LncRNAs under abiotic stresses in broccoli.
作者
裴徐梨
焦鹏
荆赞革
杞如春
王定康
唐征
PEI Xuli;JIAO Peng;JING Zange;QI Ruchun;WANG Dingkang;TANG Zheng(College of Agriculture and Life Sciences,Kunming University,Kunming 650214,China;College of Agriculture and Biotechnology,Wenzhou Vocational College of Science and Technology,Wenzhou Zhejiang 325006,China)
出处
《西北农业学报》
CAS
CSCD
北大核心
2023年第4期593-599,共7页
Acta Agriculturae Boreali-occidentalis Sinica
基金
云南省科技厅基础研究专项青年项目(2019-1-C-25318000002236)
浙江省自然科学基金(LY18C150006)
云南省地方本科高校(部分)基础研究联合专项(2018FH001-044)
浙江省科技厅项目(2021C02065-4-2)
温州市科技项目(2019ZX007-2)。
