摘要
LTR反转录转座子是植物基因组中重要的组成部分,可作为遗传工具在生物学研究上发挥重要作用。为探究毛竹中具有潜在活性的LTR反转录转座子,解析LTR反转录转座子转座激活机制,本研究从毛竹基因组中克隆1条典型的LTR反转录转座子序列,命名为PHRE8,系统地分析了PHRE8的结构特征、插入特性和进化关系,并利用荧光定量PCR检测PHRE8在DNA甲基化抑制剂(5-氮杂胞苷)、辐射、高温、低温、高盐逆境胁迫下的转录水平。结果表明,PHRE8属于Ty3-gypsy超家族,全长5296 bp,具有完整的GAG与POL结构域,插入时间约为123.07万年,是具有理论潜在活性的转座子;在不同胁迫处理下,与野生实生苗相比,PHRE8的相对表达量均有所增加,猜测PHRE8在不同胁迫条件下,会激发其转录活性,影响宿主基因组结构和基因表达模式的变化,以适应外界环境改变。本研究结果为进一步探究毛竹基因组中活性转座子的功能奠定了一定的理论基础。
The LTR retrotransposon is an important component of the plant genome and plays an important role in biological research as a genetic tool.In order to explore potentially active LTR retrotransposon in Phyllostachys edulis and analyze the transposition activation mechanism of LTR retrotransposon,a typical LTR retrotransposon sequence from Phyllostachys edulis genome named PHRE8(Phyllostachys edulis retrotransposon 8)was cloned in this study,its structural characteristics,insertion characteristics as well as evolutionary relationship were systematically analyzed,and the transcription level of PHRE8 under DNA methylation inhibitor(5-azacytidine),irradiation,high temperature,low temperature and high salt stress was detected by fluorescent quantitative PCR.The results indicate that the PHRE8 belongs to the Ty3-gypsy superfamily,has a full length of 5296 bp with a complete GAG and POL domain and its insertion time is about 1230700 years.PHRE8 is a theoretically active transposon.Compared with wild seedlings,the relative expression of PHRE8 under different non-biotic stress increase.It is speculated that PHRE8 will stimulate transcriptional activity under different stress conditions,which may affect the changes of host genome structure and gene expression patterns to adapt to changes in the external environment.The identification and transcriptional pattern analysis of PHRE8 laid the theoretical foundation for further exploration of active transposons in Phyllostachys edulis.
作者
季航
周明兵
蒋政勤
郑浩
徐芷馨
JI Hang;ZHOU Mingbing;JIANG Zhengqin;ZHENG Hao;XU Zhixin(State Key Laboratory of Subtropical Silviculture,Zhejiang A&F University,Hangzhou,Zhejiang 311300;Zhejiang Provincial Collaborative Innovation Center for Bamboo Resources and High-Efficiency Utilization,Zhejiang A&F University,Hangzhou,Zhejiang 311300)
出处
《核农学报》
CAS
CSCD
北大核心
2020年第4期705-713,共9页
Journal of Nuclear Agricultural Sciences
基金
国家自然科学基金项目(31870656、31470615)
浙江自然科学基金重点项目(LZ19C160001)
浙江省大学生科技创新活动计划暨新苗人才计划资助项目(2018R412004).
