摘要
杂种雄性不育系是培育多系法杂交稻理想的遗传工具;杂种雄性不育性一般发生在水稻种间或亚种间,而鲜见于亚种内的品种间.本文报道了一份籼籼交杂种雄性不育系9814HS-1.田间观察结果显示, 9814HS-1在长沙10月初起或三亚3月中旬前抽穗则花粉彻底败育,结实率为0.00%,而同期抽穗的双亲T98B和M114B的育性正常.分子标记分析发现T98B和M114B的籼稻基因型频率分别达到了0.94和1.00;进一步分析注意到,它们与8个籼稻品种杂交的F1株系结实全部正常,结实率达到了80.35%~91.87%,而与4个粳稻品种的杂交后代均高度不育,表明9814HS-1的双亲都具有籼稻属性.测恢试验发现, 9814HS-1的育性能被所测验的6个籼稻品种全部恢复(结实率达到了85.24%~93.21%),表明9814HS-1具有"三交种"育种利用潜力.遗传分析显示, T98B和M114B的正反交F2和BC1F1群体中不育株与可育株的理论分离比都为1:3,推断9814HS-1的育性受两个非等位的杂合态核基因互作所控制.籼型杂种雄性不育系9814HS-1的发现为重新认识水稻生殖障碍提供了启示,对于建立杂种优势利用新途径具有重要意义.
Hybrid rice has generally a yield-increasing heterosis of 10%-15% compared with inbred parents. Food and Agriculture Organization(FAO) has recommended hybrid rice as one of the best choices for solving the food shortage in the developing countries. Male sterility provides a basic genetic tool for hybrid rice breeding. Hybrid male sterility refers to the phenomenon that F1 hybrid from two parents is abnormal or nonfunctional in the development of male organs. A new germplasm source of hybrid male sterility is valuable for establishing a novel system for using heterosis by hybrid breeding of three-way crossing, which helps to develop elite hybrid rice with better comprehensive traits.To date, hybrid male sterility occurs commonly from the crosses between species(e.g. Oryza rufipogon × Oryza sativa)or subspecies(e.g. indica rice × japonica rice). Nevertheless, it is not clear if the hybrid male sterility could be induced from a cross within a subspecies. Here, we reported firstly the hybrid male sterility resulted from a cross between two indica rice parents, i.e., two WA-type maintainer lines of indica rice T98B and M114B. The In Del marker analysis showed that T98B and M114B had an indica-genotype frequency at 0.94 and 1.00, respectively, indicating both of them belong to indica rice. Field observation showed that 9814HS-1, the F1 hybrid of T98B(as female parent) and M114B(as male parent), was eco-typically male sterile. The pollens of 9814HS-1 were completely aborted, resulting in a seed setting rate at 0.00% when heading after early October at Changsha or before mid-March at Sanya, while both parents T98B and M114B were normally fertile under the same conditions. Interestingly, when T98B and M114B were crossed with eight other indica rice varieties, all of their F1 hybrids were normally fertile with a seed setting rate ranged from 80.35% to 91.87%, implying that the hybrid male sterility of 9814HS-1 is induced from the specific parental incompatibility between T98B and M114B.The restorability test showed that
作者
谭炎宁
袁定阳
段美娟
李哲理
孙学武
邓华凤
袁隆平
Yanning Tan;Dingyang Yuan;Meijuan Duan;Zheli Li;Xuewu Sun;Huafeng Deng;Longping Yuan(State Key Laboratory of Hybrid Rice,Hunan Hybrid Rice Research Center,Changsha 410125,China;College of Agronomy Sciences,Hunan Agricultural University,Changsha 410128,China)
出处
《科学通报》
EI
CAS
CSCD
北大核心
2019年第31期3204-3212,共9页
Chinese Science Bulletin
基金
湖南杂交水稻研究中心科研创新基金(YB201907)
湖南农业科技创新资金(2017XC09)
湖南省自然科学基金(2019JJ40206)
湖南省科技人才托举工程(2019TJ-Q08)资助
关键词
水稻
杂种雄性不育系
杂种优势利用
多系法杂交稻
9814HS-1
Rice(Oryzae sative L.)
hybrid male sterile line(HMS line)
heterosis utilization
multiple-lines hybrid rice
9814HS-1
