摘要
[目的]探明氟啶虫酰胺在蛋鸡中的代谢产物及代谢途径,采用放射性同位素14C标记的方法进行氟啶虫酰胺在蛋鸡中的代谢试验。[方法]制备给药胶囊,连续7 d经口给予胶囊1粒/d,最后1次给药后约8 h进行解剖。鸡蛋、肝脏与肌肉样品经过乙腈和乙腈-水溶液提取,对提取液进行残留分析。采用高效液相色谱-在线同位素检测仪(HPLC-ARC)对提取液进行放射性组分分析,采用高效液相色谱-串联质谱(HPLC-MS/MS)对代谢产物进行结构确证。[结果]氟啶虫酰胺在可食用组织肝脏及肌肉中的含量较低,在鸡蛋提取液中质量分数为1.31 mg/kg,肝脏提取液中质量分数0.453 mg/kg,肌肉提取液中质量分数为0.246 mg/kg。[结论]根据鸡蛋、肝脏和肌肉的放射性组分分析结果可以推断氟啶虫酰胺在蛋鸡体内能够被迅速代谢,可食性组织中主要以代谢产物4-(三氟甲基)烟酰胺的形式存在,在蛋鸡体内的主要代谢途径为N-去烷基化。
[Aims] This study aims to explore the metabolites and metabolic pathways of flonicamid in laying hens,the metabolic tests of flonicamid in laying hens were carried out by radioisotope14C labeling method.[Methods] Administration capsules were prepared, each laying hen was orally administered one capsule a day for7 consecutive day. Anatomy was performed about 8 h after the last administration. Egg, liver and muscle samples were extracted with acetonitrile and acetonitrile-aqueous solution. The radioactive components of the extract were analyzed with high performance liquid chromatography-on-line isotope detector(HPLC-ARC), and the metabolites were confirmed by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS). [Results] The analysis results showed that flonicamid content in edible tissues of liver and muscle is low, the mass concentration in egg extract was 1.31 mg/kg, the mass concentration in liver extract was 0.453 mg/kg, and the mass concentration in muscle extract was 0.246 mg/kg. [Conclusions] According to analysis results of the eggs, liver and muscle radioactive components can be concluded that flonicamid in laying hens can be rapidly in vivo metabolized, 4-(trifluoromethyl)nicotinamide was the mainly metabolites, the major metabolic pathways in laying hens was N-dealalkylation.
作者
孙慧
王泽华
王喜乐
王帅
刘永利
黄伟
余洋
SUN Hui;WANG Ze-hua;WANG Xi-le;WANG Shuai;LIU Yong-li;HUANG Wei;YU Yang(Safety Evaluation Center,Shenyang SYRICI Testing Co.,Ltd.,Shenyang 110141,China)
出处
《农药》
CAS
CSCD
北大核心
2023年第2期129-133,共5页
Agrochemicals
关键词
氟啶虫酰胺
同位素标记
蛋鸡
代谢物
高效液相色谱-串联质谱
flonicamid
isotope labeling
laying hen
metabolite
high-performance liquid chromatography-tandem mass spectrometry
