摘要
建立复元胶囊中五味子醇甲的含量测定方法。采用高效液相色谱(HPLC)法对复元胶囊中五味子主要成分五味子醇甲(C_(24)H_(32)O_(7))进行含量测定;流动相为甲醇-水(65∶35),检测波长为250 nm。五味子醇甲(C_(24)H_(32)O_(7))在4.096~40.96μg/mL范围内溶液浓度与峰面积存在良好的线性关系,定量限为0.399μg/mL,检测限为0.120μg/mL,平均回收率为94.4%,RSD为0.6%(n=6);精密度试验、重复性试验、稳定性试验均良好。该方法灵敏度高、准确可靠,可以有效地控制复元胶囊的质量。
A method for the determination of schisandrol A in Fuyuan capsules was established. The content of schisandrin A(C_(24)H_(32)O_(7)), as the main component of Schisandra chinensis in Fuyuan Capsules, was determined by high performance liquid chromatography(HPLC), the mobile phase was methanol-water(65∶35), and the detection wavelength was 250 nm. Schizandrin A(C24H32O7) had a good linear relationship between the solution concentration and the peak area in the range of 4.096 μg/mL to 40.96 μg/mL, the limit of quantification was 0.399 μg/mL, the limit of detection was 0.120 μg/mL, and the average recovery was 94.4%, RSD was 0.6%(n=6), the precision test, repeatability test and stability test were all good. The method has high sensitivity, accuracy and reliability, and can effectively control the quality of Fuyuan capsules.
作者
周凤盈
王殿奎
耿春辉
孙琛
王夏青
ZHOU Feng-ying;WANG Dian-kui;GENG Chun-hui;SUN Chen;WANG Xia-qing(Hengshui City Comprehensive Inspection and Testing Center,Hebei Hengshui 053000,China)
出处
《广州化工》
CAS
2022年第24期93-96,共4页
GuangZhou Chemical Industry
基金
河北省衡水市科技计划项目(No:2022014089Z)。
