摘要
目的:建立HPLC法测定复方益肝灵片中五味子醇甲的含量。方法:采用C18色谱柱,型号:SHISEIDO CAPCELL PAK C18(4.6 mm×250 mm,5μm),流动相:甲醇-水(60∶40);流速:1.0 mL·min-1,检测波长:250 nm,柱温:40℃。结果:五味子醇甲进样量在0.038～0.38μg范围内线性关系良好,其回归方程为:Y=1.720×106X+1.692×103,相关系数(r)为0.9999,精密度RSD为0.6%(n=6),重复性试验RSD为0.3%(n=6),方法回收率为101.5%(RSD=0.8%,n=6)。结论:实验结果表明该方法简便,灵敏,专属性强,重复性好,可作为复方益肝灵片的质量控制方法。
Objective：To establish an HPLC method of the determination of schizandrol A in compound Yiganling tablets. Methods：The content of schizandrol A was determined by C 18 （250 mm× 4. 6 mm ,5 μm）ODS column, the mobile phase was consisted of methanol -water （60： 40） , the flow rate was 1.0 mL · min^-1, the detection wave- length was at 286 nm, the temperature of column was 40 ℃. Results： The calibration curve of schizandrol A was linear in the range of 0. 038 - 0. 38 μg, the linear equation was Y = 1. 720 ×10^6X ＋ 1. 692×10^3, r = 0. 9999. The pre- cision （ RSD ） was 0.6% （n= 6 ）. The reproducibility （ RSD ） of this method was 0. 3 % （ n = 6）. The average recovery of schizandrol A was 101.5% （ RSD = 0. 8%, n = 6 ）. Conclusions： The method is easy, sensitive, specific, and accurate with good reproducibility. It can be used for quality control of sehizandrol A in compound Yiganling tablets.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2009年第2期295-297,共3页
Chinese Journal of Pharmaceutical Analysis
