摘要
目的研究血清长链非编码RNA核仁小RNA宿主基因5(lncRNA SNHG5)水平在评估ARDS患儿疾病严重程度及预后中的作用。方法本研究为队列研究,采取非随机抽样的方法选取湖南航天医院新生儿科新生ARDS患儿98例为研究对象,根据ARDS患儿首次胸片结果及疾病严重程度分为轻度组26例、中度组39例、重度组33例;根据患儿住院治疗期间预后情况分为预后良好组75例和预后不良组23例。采用定量反转录聚合酶连锁反应法检测血清lncRNA SNHG5水平;绘制受试者工作特征曲线分析血清lncRNA SNHG5水平预测ARDS患儿预后的价值;采用多因素logistic回归分析ARDS患儿预后不良的影响因素。结果不同疾病严重程度ARDS患儿的lncRNA SNHG5水平比较有统计学意义(F=42.51,P<0.001),其中重度组ARDS患儿血清lncRNA SNHG水平(0.27±0.09)低于中度组(0.45±0.16)和轻度组(0.64±0.20),中度组血清lncRNA SNHG5水平低于轻度组(均P<0.05)。预后不良组血清lncRNA SNHG5水平低于预后良好组[(0.29±0.09)比(0.46±0.18),t=4.35,P<0.001]。预后不良组胎膜早破比例低于预后良好组[8.70%(2/23)比38.67%(29/75)χ^(2)=7.31,P=0.007];预后不良组孕母妊娠期高血压比例高于预后良好组[26.09%(6/23)比1.33%(1/75),χ^(2)=16.26,P<0.05]。血清lncRNA SNHG5水平最佳截断点为0.34时,预测ARDS患儿预后不良的曲线下面积为0.832(95%CI:0.751~0.913),敏感度为76.0%,特异度为87.0%。lncRNA SNHG5增高是ARDS患儿预后不良的保护因素(95%CI:0.725~0.954,P=0.009)。结论血清lncRNA SNHG5水平降低与ARDS患儿病情进展及预后不良有关,且对评估预后有价值。
Objective To study the role of serum long non-coding RNA small nucleolar RNA host gene 5(lncRNA SNHG5)in assessing disease severity and in prognosis in children with acute respiratory distress syndrome(ARDS).Methods This was a cohort study.Non-random sampling method was used.Ninety-eight children with ARDS in the Newborn Department of Hunan Aerospace Hospital were selected as study subjects.According to the first chest radiograph results of children with ARDS and the severity of the disease,they were divided into mild group(26 cases),moderate group(39 cases),and severe group(33 cases);according to the prognosis during hospitalization,the children were classified into good prognosis group(75 cases)and poor prognosis group(23 cases).qRT-PCR was used to measure the serum lncRNA SNHG5 level;ROC curve was drawn to analyze the value of serum lncRNA SNHG5 level in predicting the prognosis of children with ARDS;multivariate Logistic regression was used to analyze the influencing factors of poor prognosis in children with ARDS.Results The level of lncRNA SNHG in children with ARDS of different disease severity was statistically significant(F=42.51,P<0.001).The level of serum lncRNA SNHG in severe group(0.27±0.09)was lower than that in moderate group(0.45±0.16)and mild group(0.64±0.20),with the level in moderate group lower than that in mild group(P<0.05).The level of serum lncRNA SNHG5 in poor prognosis group(0.29±0.09)was lower than that in good prognosis group(0.46±0.18)(t=4.35,P<0.001).The proportion of premature rupture of membranes in poor prognosis group was lower than that in good prognosis group,with 8.70%(2/23)vs 38.67%(29/75),(χ^(2)=7.31,P=0.007).The proportion of pregnancy induced hypertension in poor prognosis group was 26.09%(6/23),higher than that in good prognosis group 1.33%(1/75),(χ^(2)=16.26,P<0.05).When the optimal cut-off point of serum lncRNA SNHG5 level was 0.34,the area under the curve to predict the poor prognosis of children with ARDS was 0.832(95%CI:0.751-0.913),the sensitivity was 76.0%,a
作者
廖建兰
朱灿辉
熊海玲
刘腊梅
夏美芬
Liao Jianlan;Zhu Canhui;Xiong Hailing;Liu Lamei;Xia Meifen(Department of Neonatology,Hunan Aerospace Hospital,Changsha 410000,China)
出处
《国际呼吸杂志》
2023年第1期63-67,共5页
International Journal of Respiration
关键词
呼吸窘迫综合征
婴儿
新生
基因
预后
Respiratory distress syndrome
Infant,new-born
Genes
Prognosis
