摘要
为建立一种能在固定化漆酶的同时使其充分发挥酶活性从而实现染料高效降解方法,采用溶胶凝胶方法将漆酶包埋固定化在海藻酸铜(SA)或掺杂膨润土的海藻酸铜(SA/BT)凝胶小球中。通过调节单因素实验优化制备条件,采用SEM、FT-IR、XRD等表征包埋漆酶后膨润土掺杂海藻酸铜球的微观结构形貌。以活性蓝19作为目标染料降解物研究SA和SA/BT的染料降解性能,结果表明:包埋漆酶的最佳条件为20 mg/L的海藻酸钠、0.15 mol/L的氯化铜和16 g/L的膨润土;膨润土的添加能够有效提高海藻酸盐铜球的机械强度。2种固定化漆酶均表现出良好的染料降解性能:对活性蓝19的降解效率在120 min后分别达到了89.6%和91.0%;SA/BT在循环5次后,对活性蓝19的降解效率仍达到60%以上。因此,膨润土掺杂海藻酸铜球包埋漆酶能够有效提高酶稳定性和降解效率。
The laccase was embedded and immobilized in alginate or bentonite-doped alginate gel beads by sol-gel method, The aim is to establish a method that can immobilize laccase while fully exerting its enzymatic activity to achieve efficient degradation of dyes. The preparation conditions were optimized by adjusting the single factor experiment, and the microstructure and morphology of the bentonite-doped copper alginate spheres embed laccase were characterized by SEM, FT-IR and XRD. The dye degradation properties of SA@laccase and SA/BT@laccase were determined with reactive blue 19 as the target degradant. The results showed that the optimal conditions for embedding laccase were 20 mg/L sodium alginate, 0.15 mol/L copper chloride and 16 g/L bentonite;the addition of bentonite could effectively improve the mechanical strength of alginate copper spheres. Both immobilized laccases show good dye degradation performance, and the degradation efficiency of reactive blue 19 reached 89.6% and 91.0% after 120 min, respectively. The degradation efficiency of SA/BT@laccase to reactive blue 19 can still reach more than 60% after 5 cycles.
作者
黄新健
陶喜洋
武占省
田飞
HUANG Xinjian;TAO Xiyang;WU Zhansheng;TIAN Fei(School of Environmental and Chemical Engineering/Xi’an Laboratory of Textile Chemical Engineering Auxiliaries,Xi’an Polytechnic University,Xi’an 710048,China)
出处
《纺织高校基础科学学报》
CAS
2023年第1期18-25,35,共9页
Basic Sciences Journal of Textile Universities
基金
国家自然科学基金(21868034)。
关键词
膨润土
海藻酸钠
氯化铜
漆酶
固定化
bentonite
sodium alginate
copper chloride
laccase
immobilization
