摘要
旨在揭示白羽肉鸡孵化性状的遗传基础。本试验以白羽肉鸡A、B两个公鸡群体为素材,测定A群体5个世代(556只)、B群体3个世代(398只)的40周龄种蛋受精率和孵化率,并利用55K SNP芯片对两个群体共954个健康个体进行基因分型。基于系谱和基因型信息计算的A群体的受精率遗传力分别为0.21和0.14。利用混合线性模型对受精率和孵化率进行全基因组关联分析(genome-wide association study,GWAS)。全基因组关联分析共关联到12个显著SNPs,分布在1、9、11、12、20号染色体上,其中有9个SNPs与受精率显著关联,有3个SNPs与孵化率显著关联,对A群体受精率高、低组各4只公鸡睾丸组织进行转录组测序,实时荧光定量PCR验证两个差异表达基因。全基因组关联分析筛选出9个与受精率相关的候选基因COPG1、ADAMTS18、FABP2、CDH8、SLCO4A1、ATP13A3、NELL2、VEZT和IGHMBP2;3个与孵化率相关的候选基因TMEM、SCO1和MYH1A。转录组测序与荧光定量PCR验证了两个与受精率相关的候选基因HMGCLL1和COA6。本研究利用GWAS和转录组测序筛选到孵化性状的相关候选基因,为白羽肉鸡孵化性状的遗传改良提供了重要的分子标记。
The study aimed to reveal the genetic basis of hatching traits of white-feathered broilers. In this experiment, two groups of White-feather broilers A and B were used as materials to determine the fertilization rate and hatching rate of 40-week-old eggs in 5 generations(556) of group A and 3 generations(398) of group B. The 55K SNP chip was used to genotype 954 healthy individuals in the two groups. Genetic parameter estimation based on pedigree and single nucleotide polymorphisms(SNPs) showed that the heritability of fertilization rate of group A was 0.21 and 0.14, respectively. Genome-wide association study(GWAS) of fertilization rate and hatching rate was performed using mixed linear model. A total of 12 significant SNPs were obtained by genome-wide association study, distributed on chromosome 1, 9, 11, 12, 20. Among them, 9 SNPs were significantly associated with fertilization rate, 3 SNPs were significantly associated with hatching rate, RNA-seq was performed on testis tissues of 4 roosters in each group with high or low fertilization rate, 2 differentially expressed genes were identified by qPCR. Nine genes related to fertilization rate were identified by genome-wide association study, including COPG1, ADAMTS18, FABP2, CDH8, SLCO4A1, ATP13A3, NELL2, VEZT and IGHMBP2,3 genes related to hatching rate, including TMEM, SCO1 and MYH1A, RNA-seq and qPCR identified two candidate genes, HMGCLL1 and COA6, which were related to fertilization rate. In this study, GWAS and RNA-seq were used to identified candidate genes related to hatching traits, which provided important molecular markers for the genetic improvement of hatching traits of White-feathered broilers.
作者
张高猛
丁纪强
刘昱宏
郑麦青
文杰
赵桂苹
李庆贺
ZHANG Gaomeng;DING Jiqiang;LIU Yuhong;ZHENG Maiqing;WEN Jie;ZHAO Guiping;LI Qinghe(Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China)
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2023年第2期534-544,共11页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
广东省重点领域研发计划项目(2020B020222002)。
