摘要
目的 探讨宫颈癌患者伴人乳头瘤病毒(HPV)与单纯宫颈癌患者血清微小RNA(miR)-18a, miR-485-5p表达差异,分析二指标对HPV阳性宫颈癌的诊断价值。方法 选取2020年1月-2021年1月黄河三门峡医院收治的91例HPV阳性宫颈癌患者为HPV阳性宫颈癌组,62例HPV阳性非宫颈癌妇女为HPV阳性组,57名体检健康妇女为对照组,采用qRT-PCR检测血清miR-18a、miR-485-5p的表达。采用受试者操作特征曲线(ROC)分析血清miR-18a、miR-485-5p对HPV阳性宫颈癌的诊断价值。结果 HPV阳性宫颈癌组血清miR-18a表达高于对照组和HPV阳性组,而miR-485-5p表达低于对照组和HPV阳性组(均P<0.05)。血清miR-18a、miR-485-5p表达与HPV阳性宫颈癌患者FIGO分期、间质浸润深度、是否淋巴结转移有关(均P<0.05)。ROC曲线显示,miR-18a、miR-485-5p、联合诊断HPV阳性宫颈癌的AUC分别为0.752、0.802、0.876,灵敏度分别为67.03%、75.82%、83.52%,特异度分别为77.42%、74.19%、83.87%。联合诊断HPV阳性宫颈癌的AUC大于miR-18a、miR-485-5p单独诊断(均P<0.05)。结论 HPV阳性宫颈癌血清miR-18a高表达,miR-485-5p低表达,可作为HPV阳性宫颈癌辅助诊断指标。
Objective To explore the difference of serum MicroRNA(miR)-18a and miR-485-5p expression between cervical cancer patients with human papillomavirus(HPV) and simple cervical cancer patients, and analyze the diagnostic value of the two indicators for HPV-positive cervical cancer. Methods A total of 91 patients with HPV-positive cervical cancer admitted to Yellow River Sanmenxia Hospital from January 2020 to January 2021 were selected as the HPV-positive cervical cancer group. Sixty-two women with HPV-positive non-cervical cancer were placed in the HPV-positive group, and 57 healthy women based on physical examination were placed in the control group. Serum miR-18a and miR-485-5p expression was detected by qRT-PCR. Receiver operating characteristic(ROC) curve was used to analyse the diagnostic value of two indicators for HPV-positive cervical cancer. Results The expression of serum miR-18a in HPV-positive cervical cancer group was higher, while the expression of miR-485-5p was lower than those in the control group and HPV-positive cervical cancer group(P<0.05). Serum miR-18a and miR-485-5p expression was associated with FIGO stage, depth of interstitial infiltration and lymph node metastasis in patients with HPV-positive cervical cancer(P<0.05). The ROC curves showed that the area under the curve(AUC) for miR-18a and miR-485-5p and their combination for the diagnosis of HPV-positive cervical cancer were 0.752, 0.802 and 0.876, respectively, with sensitivities of 67.03%, 75.82% and 83.52%, and specificities of 77.42%, 74.19% and 83.87%, respectively. The AUC of the combined diagnosis of HPV-positive cervical cancer was greater than that of miR-18a and miR-485-5p alone(P<0.05). Conclusion High expression of serum miR-18a and low expression of miR-485-5p in HPV-positive cervical cancer can be used as an auxiliary diagnostic indicator for HPV-positive cervical cancer.
作者
李丹萍
李宗民
张丽峡
杨伟伟
李巧莉
LI Dan-ping;LI Zong-min;ZHANG Li-xia;YANG Wei-wei;LI Qiao-li(Department of Gynaecology,Yellow River Sanmenxia Hospital,Sanmenxia,Henan 472000,China;不详)
出处
《中华全科医学》
2023年第2期263-266,共4页
Chinese Journal of General Practice
基金
河南省科技发展计划项目(182102510069)。
